Extremely low frequency electromagnetic radiation enhanced energy metabolism and induced oxidative stress in Caenorhabditis elegans

Abstract

Extremely low frequency electromagnetic radiation enhanced energy metabolism and induced oxidative stress in Caenorhabditis elegans Sun YY, Wang YH, Li ZH, Shi ZH, Liao YY, Tang C, Cai P. [Extremely low frequency electromagnetic radiation enhanced energy metabolism and induced oxidative stress in Caenorhabditis elegans]. [Article in Chinese] . Sheng Li Xue Bao. 2019 Jun 25;71(3):388-394. Abstract The aim of this study was to determine the effects of extremely low frequency electromagnetic field (ELF-EMF) on energy metabolism and oxidative stress in Caenorhabditis elegans (C. elegans). Worms in three adult stages (young adult stage, egg-laying stage and peak egg-laying stage) were investigated under 50 Hz, 3 mT ELF-EMF exposure. ATP levels, ATP synthase activity in vivo, reactive oxygen species (ROS) content, and changes of total antioxidant capacity (TAC) were detected, and worms' oxidative stress responses were also evaluated under ELF- EMF exposure. The results showed that ATP levels were significantly increased under this ELF-EMF exposure, and mitochondrial ATP synthase activity was upregulated simultaneously. In young adult stage, worms' ROS level was significantly elevated, together with upregulated TAC but with a decreased ROS-TAC score indicated by principal component analysis. ROS level and TAC of worms had no significant changes in egg-laying and peak egg-laying stages. Based on these results, we concluded that ELF-EMF can enhance worm energy metabolism and elicit oxidative stress, mainly manifesting as ATP and ROS level elevation together with ATP synthase upregulation and ROS-TAC score decrease in young adult C. elegans. ncbi.nlm.nih.gov

AI evidence extraction

Main findings

Under 50 Hz, 3 mT ELF-EMF exposure, ATP levels were significantly increased and mitochondrial ATP synthase activity was upregulated. In young adult worms, ROS was significantly elevated and TAC was upregulated, with a decreased ROS-TAC score; in egg-laying and peak egg-laying stages, ROS and TAC showed no significant changes.

Outcomes measured

• ATP levels
• Mitochondrial ATP synthase activity (in vivo)
• Reactive oxygen species (ROS) content
• Total antioxidant capacity (TAC)
• Oxidative stress responses
• ROS-TAC score (principal component analysis)

Limitations

• Exposure duration not reported in the abstract
• Sample size not reported in the abstract
• Details of oxidative stress response assays and statistical methods are not provided in the abstract

Suggested hubs

• elF-oxidative-stress (0.78)
  ELF-EMF exposure assessed for ROS/TAC and oxidative stress outcomes in an animal model.

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        "source": null,
        "frequency_mhz": 0.05000000000000000277555756156289135105907917022705078125,
        "sar_wkg": null,
        "duration": null
    },
    "population": "Caenorhabditis elegans (young adult, egg-laying, peak egg-laying stages)",
    "sample_size": null,
    "outcomes": [
        "ATP levels",
        "Mitochondrial ATP synthase activity (in vivo)",
        "Reactive oxygen species (ROS) content",
        "Total antioxidant capacity (TAC)",
        "Oxidative stress responses",
        "ROS-TAC score (principal component analysis)"
    ],
    "main_findings": "Under 50 Hz, 3 mT ELF-EMF exposure, ATP levels were significantly increased and mitochondrial ATP synthase activity was upregulated. In young adult worms, ROS was significantly elevated and TAC was upregulated, with a decreased ROS-TAC score; in egg-laying and peak egg-laying stages, ROS and TAC showed no significant changes.",
    "effect_direction": "mixed",
    "limitations": [
        "Exposure duration not reported in the abstract",
        "Sample size not reported in the abstract",
        "Details of oxidative stress response assays and statistical methods are not provided in the abstract"
    ],
    "evidence_strength": "low",
    "confidence": 0.7800000000000000266453525910037569701671600341796875,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "extremely low frequency",
        "ELF-EMF",
        "50 Hz",
        "3 mT",
        "Caenorhabditis elegans",
        "energy metabolism",
        "ATP",
        "ATP synthase",
        "oxidative stress",
        "ROS",
        "total antioxidant capacity",
        "TAC"
    ],
    "suggested_hubs": [
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