Pulsed magnetic field improves seed quality of aged green pea seeds by homeostasis of free radical content

Abstract

Pulsed magnetic field improves seed quality of aged green pea seeds by homeostasis of free radical content Bhardwaj J, Anand A, Pandita VK, Nagarajan S. Pulsed magnetic field improves seed quality of aged green pea seeds by homeostasis of free radical content. J Food Sci Technol. 2016 Nov;53(11):3969-3977. doi: 10.1007/s13197-016-2392-8. Abstract To elucidate the mechanism responsible for magnetic field induced seed invigoration in aged seeds an experiment was conducted on six year old garden pea seeds stored under controlled (20 °C and 40% RH) condition. Aged seeds were magnetoprimed by exposing to pulsed magnetic field (PMF) of 100 mT for 1 h in three pulsed modes. The 6 min on and off PMF showed significant improvement in germination (7.6%) and vigor (84.8%) over aged seeds. Superoxide and hydrogen peroxide production increased in germinating primed seeds by 27 and 52%, respectively, over aged seeds. Nicotinamide adenine dinucleotide (reduced) (NADH) peroxidase and superoxide dismutase involved in generation of hydrogen peroxide showed increased activity in PMF primed seeds. Increase in catalase, ascorbate peroxidase and glutathione reductase activity after 36 h of imbibition in primed seeds demonstrated its involvement in seed recovery during magnetopriming. An increase in total antioxidants also helped in maintaining the level of free radicals for promoting germination of magnetoprimed seeds. A 44% increase in level of protein carbonyls after 36 h indicated involvement of protein oxidation for counteracting and/or utilizing the production of ROS and faster mobilization of reserve proteins. Higher production of free radicals in primed seeds did not cause lipid peroxidation as malondialdehyde content was low. Lipoxygenase was involved in the germination associated events as the magnitude of activity was higher in primed aged seeds compared to aged seeds. Our study elucidated that PMF mediated improvement in seed quality of aged pea seeds was facilitated by fine tuning of free radicals by the antioxidant defense system and protein oxidation. ncbi.nlm.nih.gov

AI evidence extraction

Main findings

Aged pea seeds exposed to a pulsed magnetic field (100 mT for 1 h) showed improved germination and vigor, with the 6 min on/off pulsed mode reported as significantly improving germination (7.6%) and vigor (84.8%) versus aged seeds. Primed seeds showed increased superoxide and hydrogen peroxide production and increased activities of several antioxidant/ROS-related enzymes; lipid peroxidation (malondialdehyde) remained low.

Outcomes measured

• Germination
• Seed vigor
• Superoxide production
• Hydrogen peroxide production
• NADH peroxidase activity
• Superoxide dismutase activity
• Catalase activity
• Ascorbate peroxidase activity
• Glutathione reductase activity
• Total antioxidants
• Protein carbonyls
• Malondialdehyde (lipid peroxidation marker)
• Lipoxygenase activity

Limitations

• Sample size not reported in the abstract
• Only aged pea seeds studied; generalizability to other species/conditions not stated
• Exposure parameters beyond field strength, duration, and pulsed mode (e.g., pulse frequency/waveform) not provided in the abstract

{
    "study_type": "animal",
    "exposure": {
        "band": null,
        "source": "pulsed magnetic field (magnetopriming)",
        "frequency_mhz": null,
        "sar_wkg": null,
        "duration": "1 h"
    },
    "population": "Six-year-old garden pea (green pea) seeds stored at 20 °C and 40% RH",
    "sample_size": null,
    "outcomes": [
        "Germination",
        "Seed vigor",
        "Superoxide production",
        "Hydrogen peroxide production",
        "NADH peroxidase activity",
        "Superoxide dismutase activity",
        "Catalase activity",
        "Ascorbate peroxidase activity",
        "Glutathione reductase activity",
        "Total antioxidants",
        "Protein carbonyls",
        "Malondialdehyde (lipid peroxidation marker)",
        "Lipoxygenase activity"
    ],
    "main_findings": "Aged pea seeds exposed to a pulsed magnetic field (100 mT for 1 h) showed improved germination and vigor, with the 6 min on/off pulsed mode reported as significantly improving germination (7.6%) and vigor (84.8%) versus aged seeds. Primed seeds showed increased superoxide and hydrogen peroxide production and increased activities of several antioxidant/ROS-related enzymes; lipid peroxidation (malondialdehyde) remained low.",
    "effect_direction": "benefit",
    "limitations": [
        "Sample size not reported in the abstract",
        "Only aged pea seeds studied; generalizability to other species/conditions not stated",
        "Exposure parameters beyond field strength, duration, and pulsed mode (e.g., pulse frequency/waveform) not provided in the abstract"
    ],
    "evidence_strength": "low",
    "confidence": 0.7399999999999999911182158029987476766109466552734375,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "pulsed magnetic field",
        "magnetopriming",
        "pea seeds",
        "seed invigoration",
        "germination",
        "vigor",
        "reactive oxygen species",
        "antioxidant enzymes",
        "protein oxidation",
        "lipid peroxidation"
    ],
    "suggested_hubs": []
}

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