Electromagnetic field-induced adaptive response in Schwann cells through DNA methylation, histone

Abstract

Electromagnetic field-induced adaptive response in Schwann cells through DNA methylation, histone deacetylation, and oxidative stress Colciago A, Mohamed T, Colleoni D, Melfi V, Magnaghi V. Electromagnetic field-induced adaptive response in Schwann cells through DNA methylation, histone deacetylation, and oxidative stress. J Cell Physiol. 2024 Jun 30:e31365. doi: 10.1002/jcp.31365. Abstract Schwannomas are benign tumors of the peripheral nervous system arising from the transformation of Schwann cells (SCs). On the whole, these tumors are related to alterations of the neurofibromin type 2 gene, coding for the oncosuppressor merlin, a cytoskeleton-associated protein belonging to the ezrin- radixin-moesin family. However, the underlying mechanisms of schwannoma onset and progression are not fully elucidated, whereas one of the challenges might be the environment. In this light, the exposure to electromagnetic field (EMF), generated by the use of common electrical devices, has been defiantly suggested as the cause of SCs transformation even if the evidence was mostly epidemiologic. Indeed, insubstantial mechanisms have been so far identified to explain SCs oncotransformation. Recently, some in vitro evidence pointed out alterations in proliferation and migration abilities in SCs exposed to EMF (0.1 T, 50 Hz, 10 min). Here, we used the same experimental paradigma to discuss the involvement of putative epigenetic mechanisms in SCs adaptation to EMF and to explain the occurrence of hypoxic alterations after the exposure. Our findings indicate a set of environmental-induced changes in SCs, toward a less-physiological state, which may be pathologically relevant for the SCs differentiation and the schwannoma development. pubmed.ncbi.nlm.nih.gov

AI evidence extraction

Main findings

Using an in vitro exposure paradigm (0.1 T, 50 Hz, 10 min), the authors report environmental-induced changes in Schwann cells involving putative epigenetic mechanisms (DNA methylation and histone deacetylation) and oxidative stress, and discuss these in relation to hypoxic alterations after exposure. They conclude these changes shift Schwann cells toward a less-physiological state that may be pathologically relevant for Schwann cell differentiation and schwannoma development.

Outcomes measured

• Epigenetic mechanisms (DNA methylation, histone deacetylation)
• Oxidative stress
• Hypoxic alterations
• Cell differentiation state / less-physiological state
• Schwannoma development relevance (pathological relevance)

Limitations

• Sample size not reported in abstract
• Specific quantitative results and statistical details not provided in abstract
• In vitro model; relevance to human exposure and schwannoma risk not established in abstract

Suggested hubs

• mechanisms-epigenetics (0.86)
  Focus on DNA methylation and histone deacetylation as mechanisms after EMF exposure.
• elf-emf (0.84)
  Exposure specified as 50 Hz, 0.1 T (ELF magnetic field).
• oxidative-stress (0.78)
  Oxidative stress is highlighted as part of the adaptive response.

{
    "study_type": "in_vitro",
    "exposure": {
        "band": "ELF",
        "source": "common electrical devices",
        "frequency_mhz": 0.05000000000000000277555756156289135105907917022705078125,
        "sar_wkg": null,
        "duration": "10 min"
    },
    "population": "Schwann cells (SCs)",
    "sample_size": null,
    "outcomes": [
        "Epigenetic mechanisms (DNA methylation, histone deacetylation)",
        "Oxidative stress",
        "Hypoxic alterations",
        "Cell differentiation state / less-physiological state",
        "Schwannoma development relevance (pathological relevance)"
    ],
    "main_findings": "Using an in vitro exposure paradigm (0.1 T, 50 Hz, 10 min), the authors report environmental-induced changes in Schwann cells involving putative epigenetic mechanisms (DNA methylation and histone deacetylation) and oxidative stress, and discuss these in relation to hypoxic alterations after exposure. They conclude these changes shift Schwann cells toward a less-physiological state that may be pathologically relevant for Schwann cell differentiation and schwannoma development.",
    "effect_direction": "harm",
    "limitations": [
        "Sample size not reported in abstract",
        "Specific quantitative results and statistical details not provided in abstract",
        "In vitro model; relevance to human exposure and schwannoma risk not established in abstract"
    ],
    "evidence_strength": "low",
    "confidence": 0.7399999999999999911182158029987476766109466552734375,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "Schwann cells",
        "schwannoma",
        "ELF electromagnetic field",
        "50 Hz",
        "0.1 T",
        "epigenetics",
        "DNA methylation",
        "histone deacetylation",
        "oxidative stress",
        "hypoxia"
    ],
    "suggested_hubs": [
        {
            "slug": "mechanisms-epigenetics",
            "weight": 0.85999999999999998667732370449812151491641998291015625,
            "reason": "Focus on DNA methylation and histone deacetylation as mechanisms after EMF exposure."
        },
        {
            "slug": "elf-emf",
            "weight": 0.83999999999999996891375531049561686813831329345703125,
            "reason": "Exposure specified as 50 Hz, 0.1 T (ELF magnetic field)."
        },
        {
            "slug": "oxidative-stress",
            "weight": 0.7800000000000000266453525910037569701671600341796875,
            "reason": "Oxidative stress is highlighted as part of the adaptive response."
        }
    ]
}

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