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Filters: tag: In Vitro Exposure Clear

Does Electromagnetic Pollution in the ART Laboratory Affect Sperm Quality? A Cross-Sectional Observational Study.

Research RF Safe Research Library Jan 1, 2025

This cross-sectional observational study assessed sperm motility after one hour of in vitro exposure of semen samples to EMFs from different laboratory sources in an IVF setting. It reports a statistically significant reduction in progressive sperm motility after exposure to mobile phones and Wi-Fi repeaters, while…

Effect of electromagnetic field radiation on transcriptomic profile and DNA methylation level in pig conceptuses during the peri-implantation period

Research RF Safe Research Library Jan 1, 2025

This in vitro study exposed pig conceptuses (days 15–16 of pregnancy) to 50 Hz ELF-EMF for 2 hours and assessed transcriptomic and DNA methylation changes. The authors report altered expression of 21 protein-coding transcripts and an approximately 16-fold increase in genomic DNA methylation, with promoter methylation…

First Identification of the Effects of Low Frequency Electromagnetic Field on the Micromolecular Changes in Adipose Tissue-Derived Mesenchymal Stem Cells by Fourier Transform Infrared Spectroscopy.

Research RF Safe Research Library Jan 1, 2021

This in vitro study exposed adipose tissue-derived mesenchymal stem cells to a continuous 50 Hz electromagnetic field at 1.5 mT for 24, 48, or 72 hours and assessed micromolecular changes using FTIR spectroscopy. The authors report the largest FTIR spectral differences versus control in nucleic acids and proteins…

Genotoxic Effects in Human Fibroblasts Exposed to Microwave Radiation.

Research RF Safe Research Library Jan 1, 2018

This in vitro study exposed human fetal and adult fibroblasts to 25 GHz microwave radiation and assessed multiple genotoxic and cellular endpoints. The authors report no evidence of direct DNA damage and no apoptosis or changes in prosurvival signalling proteins. However, they report increased total and…

The effects of different intensities, frequencies and exposure times of extremely low-frequency electromagnetic fields on the growth of Staphylococcus aureus and Escherichia coli O157:H7.

Research RF Safe Research Library Jan 1, 2015

This in vitro study examined whether extremely low-frequency electromagnetic fields (ELF-EMF) affect growth of Staphylococcus aureus and Escherichia coli O157:H7. Bacterial cultures were exposed to sinusoidal homogeneous ELF-EMF at 2 or 4 mT, with frequencies of 20, 40, or 50 Hz, for 1–6 hours, and CFU were compared…

Assessment of the neurotoxic potential of exposure to 50Hz extremely low frequency electromagnetic fields (ELF-EMF) in naïve and chemically stressed PC12 cells

Research RF Safe Research Library Jan 1, 2014

This in vitro study tested acute (30 min) and sub-chronic (48 h) 50 Hz ELF-EMF exposure up to 1000 µT in naïve and chemically stressed PC12 cells. Across assays of Ca2+ homeostasis, ROS production, and membrane integrity, the study reports no consistent or measurable neurotoxic effects under the tested conditions.…

[Time effect of sinusoidal electromagnetic field on enhancing the maturation and mineralization of osteoblasts in vitro].

Research RF Safe Research Library Jan 1, 2011

This in vitro study exposed newborn rat calvarial osteoblasts to 50 Hz sinusoidal electromagnetic fields at 1.8 mT for different daily exposure times (0.5–3.0 h). The authors report inhibited osteoblast proliferation at longer exposure times, alongside increased differentiation markers (ALP activity) and…

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