Neurotoxic effects of 3.5 GHz GSM-like RF exposure on cultured DRG neurons: a mechanistic insight into oxidative and apoptotic pathways
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RF Safe Research Library
Jan 1, 2026
This in vitro study examined strictly non-thermal, GSM-like 3.5 GHz RF-EMF exposure in cultured mouse dorsal root ganglion neurons for 1–24 hours. The authors report time-dependent reductions in cell viability alongside increased ROS and changes consistent with mitochondria-mediated apoptosis (e.g., Bax/caspase-3 up,…
Effects of Extremely Low Frequency Magnetic Field Exposure (50 Hz, 200 µT) on Cell Viability, DNA Damage and Micronucleus Formation of Human Skin Cells.
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RF Safe Research Library
Jan 1, 2026
This in vitro study tested whether 50 Hz extremely low frequency magnetic fields at 200 µT (rms) affect human skin cells (HaCaT). Using blinded, simultaneous exposure and sham-exposure for 2 or 24 hours, the authors assessed cell viability, DNA integrity, and micronucleus formation. The abstract reports no…
Exposure of human lymphocytes to sweeping-frequency extremely low frequency magnetic field
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RF Safe Research Library
Jan 1, 2025
This in vitro study exposed human umbilical cord blood lymphocytes to a sinusoidal sweeping-frequency ELF magnetic field (3–26 Hz) for 48 hours at amplitudes from 6 to 24 μT. It reports no statistically significant effects on DNA damage/repair foci or apoptosis measures overall. A non-significant trend at 8 μT showed…
Proteomic Characterization of Human Peripheral Blood Mononuclear Cells Exposed to a 50 Hz Magnetic Field
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Jan 1, 2025
This in vitro study compared proteomic profiles of PBMCs from three human donors after 24-hour exposure to a 50 Hz, 1 mT extremely low-frequency magnetic field versus unexposed cells. The abstract reports broad protein expression changes, including upregulation of proteins associated with metabolic processes and…
The Frequency of a Magnetic Field Determines the Behavior of Tumor and Non-Tumor Nerve Cell Models
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Jan 1, 2025
This in vitro study exposed glioblastoma (CT2A), neuroblastoma (N2A), and non-tumor astrocyte (C8D1A) cell models to a 100 μT magnetic field across 20–100 Hz for 24–72 hours. The abstract reports decreased viability and proliferation in the tumor cell models within a frequency window centered at 50 Hz, while…
Assessing cell viability and genotoxicity in Trigonella foenum-graecum L. exposed to 2100 MHz and 2300 MHz electromagnetic field radiations.
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Jan 1, 2025
This experimental study assessed cytotoxic and genotoxic endpoints in Trigonella foenum-graecum L. exposed to 2100 MHz and 2300 MHz EMF-r for 0.5–8 h/day. The authors report reduced root/shoot growth at longer daily exposures, increased chromosomal aberrations compared with controls, and reduced cell viability.…
Comparing the Activity of Peripheral Blood Mononuclear Cells Frozen Under Electromagnetic Field Freezing and Standard Slow-Freezing.
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RF Safe Research Library
Jan 1, 2025
This study compares PBMC cryopreservation using an electromagnetic field (EMF) freezer versus standard slow-freezing (SLF). It reports equivalent viable cell number, viability, and cell activity between methods. EMF freezing required substantially less time (0.25 h vs 3 h), which may improve operational efficiency by…
Comparison of pulsed and continuous electromagnetic field generated by WPT system on human dermal and neural cells.
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RF Safe Research Library
Jan 1, 2024
This in vitro study tested electromagnetic fields generated by a prototype wireless power transfer (wireless charging) system on four human dermal and neural cell lines. Across pulsed and continuous exposure conditions, the authors report no negative effects on morphology/cytoskeleton, viability/mitosis,…
Induction of apoptosis in B16-BL6 melanoma cells following exposure to electromagnetic fields modeled after intercellular calcium waves.
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Jan 1, 2024
This in vitro study exposed B16-BL6 melanoma cells to a time-varying EMF modeled after intercellular calcium-wave firing patterns. The abstract reports a 50.3% reduction in viable cells after a single 40-minute exposure and microscopy findings consistent with apoptosis and nuclear fragmentation versus control. The…
Effects of 2.4 GHz radiofrequency electromagnetic field (RF-EMF) on glioblastoma cells (U -118 MG).
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RF Safe Research Library
Jan 1, 2023
This in vitro study exposed glioblastoma (U-118 MG) and human embryonic kidney (Hek-293) cells to 2.4 GHz RF-EMF for 24–72 hours, with or without a dielectric screen. The authors report a significant decrease in cell viability after exposure in both cell lines. The decrease was described as greater in glioblastoma…
Assessing the effect of selenium on cyclin D1 level and nuclear factor kappa b activity in NIH/3T3 fibroblast cells at 2100 MHz electromagnetic field exposure.
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RF Safe Research Library
Jan 1, 2023
This in vitro study examined NIH/3T3 fibroblast cells exposed to 2100 MHz EMF and evaluated cell viability, cyclin D1, and NF-κB activity, with and without 200 nM selenium. The abstract reports reduced cell viability and increased cyclin D1 and NF-κB activity after EMF exposure. Selenium supplementation was reported…
Intermittent Exposure to a 16 Hz Extremely Low Frequency Pulsed Electromagnetic Field Promotes Osteogenesis In Vitro through Activating Piezo 1-Induced Ca Influx in Osteoprogenitor Cells.
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Jan 1, 2023
This in vitro study compared continuous versus intermittent 16 Hz ELF-PEMF exposure strategies in osteoprogenitor (SCP-1) cells. Intermittent exposure (10 min every 8 h) was reported to enhance cell numbers and osteogenic function relative to continuous daily exposure (30 min every 24 h). The authors report increased…
Effects of repeated exposure to 50 Hz electromagnetic field on breast cancer cells.
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RF Safe Research Library
Jan 1, 2022
This in vitro study exposed two human breast cancer cell lines to a 50 Hz ELF-EMF (20 mT) for 3 hours/day for up to four days. The authors report reduced viability and apoptosis-related findings in MDA-MB-231 cells, with no significant viability change in MCF-7 cells. Cell-cycle effects differed by cell line,…
Static and Electromagnetic Fields Differently Affect Proliferation and Cell Death Through Acid Enhancement of ROS Generation in Mesenchymal Stem Cells.
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Jan 1, 2022
This in vitro study examined how a 20 mT static magnetic field and a 20 mT 50 Hz electromagnetic field affect mesenchymal stem cells under conditions involving ROS and acidic pH. The authors report increased ROS and antioxidant-response markers (Nrf2, SOD2, GST) across SMF, EMF, and combined exposures, with…
Morphological and cytophysiological changes in selected lines of normal and cancer human cells under the influence of a radio-frequency electromagnetic field.
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Jan 1, 2021
This in vitro study exposed human fibroblasts and prostate cancer cells to 2.5 GHz RF-EMF for 24, 48, and 72 hours and assessed viability and morphology. The authors report decreased viability in fibroblasts and increased viability in prostate cancer cells after exposure, while morphology showed no significant…
EVALUATION OF BCL2 AND ITS REGULATORY MIRS, MIR-15-B AND MIR-16 EXPRESSION CHANGES UNDER THE EXPOSURE OF EXTREMELY LOW-FREQUENCY ELECTROMAGNETIC FIELDS ON HUMAN GASTRIC CANCER CELL LINE.
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Jan 1, 2021
This in vitro study examined whether extremely low-frequency electromagnetic fields (ELF-EMF) at 0.2 or 2 mT (continuous or 1.5 h on/1.5 h off) for 18 hours alter viability and gene/miRNA expression in AGS gastric cancer cells. The authors report a significant reduction in cell viability at 2 mT continuous exposure.…
The Dynamic(s) of Adipose Stem Cell System, Their Survival, and Cessation under the Influence of Electromagnetic Fields.
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Jan 1, 2021
This in vitro study examined how extremely low frequency EMF affects adipose-derived stem cell proliferation, cell cycle, viability, and death using fluorescent microscopy and flow cytometry. Under continuous exposure at 76.6 Hz and 20 T (ICR-based parameters), the authors report a statistically significant increase…
Up-regulation of miR-144 and miR-375 in the human gastric cancer cell line following the exposure to extremely low-frequency electromagnetic fields.
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RF Safe Research Library
Jan 1, 2021
This in vitro study exposed AGS human gastric cancer cells to 50 Hz ELF magnetic fields at 0.2 or 2 mT for 18 hours (continuous or intermittent). The authors report reduced cell viability versus control and up-regulation of miR-144 and miR-375 during exposure. They also report miR levels decreased 18 and 36 hours…
Effect of extremely low frequency electromagnetic field parameters on the proliferation of human breast cancer.
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RF Safe Research Library
Jan 1, 2021
This in vitro study tested how varying ELF-EMF parameters (frequency, flux density, duration, and continuous vs switching exposure) affect breast cancer and normal breast epithelial cell lines. The authors report that an optimized switching exposure pattern (7.83 ± 0.3 Hz, 1 mT) reduced viability of MDA-MB-231 cells…
Low-frequency electromagnetic field influences human oral mucosa keratinocyte viability in response to lipopolysaccharide or minocycline treatment in cell culture conditions.
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Jan 1, 2021
This in vitro study examined whether low-frequency electromagnetic field (LF-EMF) exposure affects viability of human oral mucosa keratinocytes. Apoptosis (early/late) and necrosis were measured by flow cytometry, including conditions with lipopolysaccharide (LPS) or minocycline. The authors report that LF-EMF, alone…
The effect of electromagnetic fields on survival and proliferation rate of dental pulp stem cells.
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Jan 1, 2020
This in vitro study tested whether 50 Hz extremely low-frequency electromagnetic fields (0.5 or 1 mT) affect human dental pulp stem cell survival and proliferation. Using MTT and DAPI assays after 7 days of exposure (20 or 40 min/day), all exposed groups reportedly showed higher viability/proliferation than controls.…
Evaluation of Cytotoxic and Genotoxic Effects of Extremely Low-frequency Electromagnetic Field on Mesenchymal Stromal Cells.
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Jan 1, 2018
This in vitro study evaluated whether a therapeutic extremely low-frequency EMF exposure (5 Hz, 0.4 mT) causes cytotoxic or genotoxic effects in a human mesenchymal stromal cell line. Cells were exposed for 20 minutes per day, three times per week, for two weeks and assessed for viability, proliferation, and…
Extremely low frequency variable electromagnetic fields affect cancer and noncancerous cells in vitro differently: Preliminary study.
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RF Safe Research Library
Jan 1, 2018
This in vitro study exposed cancer (U87-MG; 143B) and noncancerous (BJ; HEK) cells to extremely low frequency electromagnetic fields under varying frequencies (2–60 Hz), waveforms, and exposure durations, with sham controls. Cell viability was assessed using an MTT assay. The authors report that cancer and…
RF-EMF exposure at 1800 MHz did not elicit DNA damage or abnormal cellular behaviors in different neurogenic cells.
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RF Safe Research Library
Jan 1, 2017
This in vitro study assessed whether intermittent 1800 MHz RF-EMF exposure induces DNA damage or alters cellular behavior in three neurogenic cell lines. Cells were exposed at an average SAR of 4.0 W/kg for 1, 6, or 24 hours and evaluated for γH2AX foci, cell cycle progression, proliferation, and viability. The…
Combined effects of electromagnetic field and low-level laser increase proliferation and alter the morphology of human adipose tissue-derived mesenchymal stem cells.
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RF Safe Research Library
Jan 1, 2017
This in vitro study tested 50 Hz, 3 mT electromagnetic field (EMF) exposure and 808 nm low-level laser (LLL), alone and combined, on human adipose tissue-derived mesenchymal stem cells from six donors. The authors report increased cell numbers after 7 days with EMF, greater increases with LLL, and the largest…