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272 postsEffects of Simultaneous In-Vitro Exposure to 5G-Modulated 3.5 GHz and GSM-Modulated 1.8 GHz Radio-Frequency Electromagnetic Fields on Neuronal Network Electrical Activity and Cellular Stress in Skin Fibroblast Cells
This in-vitro study exposed primary cortical neurons and human immortalized skin fibroblasts to simultaneous 5G-modulated 3.5 GHz and GSM-modulated 1.8 GHz RF-EMF at SARs of 1 or 4 W/kg. It reports no significant changes in neuronal network firing/bursting activity and no alteration of mitochondrial ROS in fibroblasts. Stress-related signaling readouts showed only minor, threshold-level variations without a consistent pattern, and no HSF1 activation was observed. Overall, the authors conclude there is no strong evidence of biological effects under these exposure conditions.
Electromagnetic Field Stimulation Effects on Intrinsically Disordered Proteins and Their Role in Aging and Neurodegeneration
This review discusses preclinical studies suggesting non-ionizing EMF exposures can produce beneficial biological effects, while noting ongoing controversy about mechanisms. It reports evidence of EMF-associated conformational changes in intrinsically disordered proteins relevant to neurodegeneration and describes RF exposure conditions that activate proteostasis and autophagy in cell and animal models. The authors propose a quantum-biophysical framework involving the water-protein interface and suggest potential human applications within regulatory safety thresholds.
RF-EMF Exposure Assessment: Comparison of Measurements in Airports and Flights with and Without Wi-Fi Service
This exposure assessment used personal exposimeters to measure RF-EMF levels in the 2.4 GHz and 5.85 GHz Wi-Fi bands in airport terminals and during four international flights, including flights with and without onboard Wi-Fi service. Reported mean exposures varied by route but were described as substantially below an international reference level (10 W/m²). The authors conclude exposure is low while also recommending ongoing monitoring and precaution due to potential health concerns mentioned as emerging evidence.
Exposure Variability Between 1- or 6-Minute and 30-Minute Averaging Time Lengths in Radiofrequency-Electromagnetic Field Exposure Monitoring
This exposure assessment study compared RF-EMF measurements averaged over 1, 6, and 30 minutes using contiguous 1-minute data collected over 30 minutes at four indoor/outdoor sites across 15 frequency bands. Relative deviations between shorter averaging times and 30-minute averages were largely within ±3 dB. However, statistical comparisons of overall exposure variability between 1- or 6-minute and 30-minute averaging produced inconsistent results, with broadcast and most mobile services <2 GHz appearing broadly similar between 1- and 6-minute averaging.
Visualizing radiofrequency electromagnetic field exposure through Voronoi-based maps
This exposure-assessment study proposes a Voronoi-diagram approach to visualize RF-EMF exposure across a city using personal exposimeter measurements of RMS electric field at seed points. Most mapped areas corresponded to about 1.9 V/m, with a maximum reported value of 11.4 V/m, all below the cited ICNIRP guideline level. The authors conclude the method is useful for communicating spatial variability, while also noting broader literature discussing potential health risks from EMF exposure.
Empowering the Serbian EMF RATEL System for Monitoring RF-EMF Through Drive Test
This engineering/monitoring paper describes enhancements to the Serbian EMF RATEL system, which has continuously monitored RF-EMF since 2017, by adding drive test functionality to improve spatial coverage. The authors report preliminary quantitative drive test measurements and validation of the upgraded approach. The work emphasizes that characterizing spatial and temporal RF-EMF patterns can support exposure assessment relevant to public health risk evaluation.
Prolonged 3.5 GHz and 24 GHz RF-EMF Exposure Alters Testicular Immune Balance, Apoptotic Gene Expression, and Sperm Function in Rats
This rat study examined 60-day RF-EMF exposure at 3.5 GHz and 24 GHz for 1 or 7 hours per day and assessed testicular cytokines, apoptosis-related gene expression, and sperm quality. The authors report changes consistent with altered immune signaling and pro-apoptotic pathways, alongside reduced sperm parameters (frequency- and duration-dependent). The conclusion frames these findings as an EMF safety concern and suggests longer daily exposure worsened negative effects.
Electromagnetic Interference in the Modern Era: Concerns, Trends, and Nanomaterial-Based Solutions
This review surveys the evolution, sources, and consequences of electromagnetic interference (EMI) in modern environments shaped by IoT, 5G, and smart devices. It discusses disruptions to electrical and medical devices, ecological impacts on wildlife, and potential risks to human health from EMI exposure. The paper emphasizes mitigation via advanced shielding materials, highlighting carbon-based nanomaterials as promising solutions.
The WHO-commissioned systematic reviews on health effects of radiofrequency radiation provide no assurance of safety
This paper evaluates and critiques 12 WHO-commissioned systematic reviews and meta-analyses on RF-EMF health effects across outcomes including cancer and reproductive endpoints. It argues that serious methodological flaws and limitations in the WHO reviews prevent them from providing assurance of safety for cell phones and other wireless devices. The authors highlight reported evidence in the animal cancer review (high certainty for heart schwannomas; moderate certainty for brain gliomas) and describe dose-related adverse effects on male fertility and reproductive outcomes, including at exposure levels below current ICNIRP thresholds.
Microwave and RF Exposure-Induced Molecular and Genetic Alterations
This review discusses recent studies on microwave and RF exposure and their reported impacts on molecular and cytogenetic materials. It states there is growing evidence that RF exposure can induce DNA damage at levels considered safe by current standards, and cites newly reported genetic alterations in rat cancers after lifetime low-level RF exposure. The article concludes that these findings challenge existing exposure guidelines and support reconsideration of regulatory limits.
Differential metabolic responses of mouse Leydig and spermatogonia cells to radiofrequency electromagnetic field exposure
This in vitro study used LC-MS metabolomics to assess how continuous versus intermittent RF-EMF irradiation affects mouse Leydig (TM3) and spermatogonia (GC-1) cells. The authors report stronger metabolic disturbances in TM3 cells under continuous exposure, including changes in amino acid and glutathione-related pathways, while intermittent exposure mainly affected fatty acyl and purine-related metabolism. GC-1 cells were reported to be less sensitive, and ADP changes were proposed as a potential metabolic signature. The authors interpret these metabolic disturbances as suggesting potential reproductive health risks.
Radiofrequency regulates the BET-mediated pathways in radial glia differentiation in human cortical development
This in vitro study reports that radiofrequency (RF) exposure in the 800–2,400 MHz range modulates differentiation pathways in human cortical organoids derived from embryonic stem cells. RF exposure is described as maintaining radial glia stem cell identity and delaying differentiation, alongside induction of endogenous retrovirus expression and increased expression of ASD-associated genes and retroelements. The abstract attributes these effects to dysregulation of BET proteins and reports that BET inhibition rescues the RF-associated developmental defects.
Investigation of fetal exposure to electromagnetic waves between 2.45 and 5 GHz during pregnancy
This dosimetry study simulated fetal RF-EMF exposure between 2.45 and 5 GHz during the second trimester, estimating SAR10g in fetal brain and lungs. The presence of a belly-button piercing increased SAR, with maxima reported at 2.45 GHz (16 mW/kg in lungs; 14 mW/kg in brain). Despite these increases, all SAR values were reported to remain below IEEE and ICNIRP limits, while the authors note a precautionary implication regarding metal objects during pregnancy.
3.5GHz radiofrequency electromagnetic fields (RF-EMF) on metabolic disorders in Drosophila melanogaster
This animal study used metabolomics to assess metabolic changes in male Drosophila melanogaster exposed to 3.5 GHz RF-EMF at 0.1, 1, and 10 W/m². It reports disruptions in four metabolic pathways and 34 differential metabolites, with significant decreases in several metabolites including GABA, glucose-6-phosphate, and AMP. The authors interpret the findings as suggesting RF-EMF-related metabolic disturbance, while noting no clear dose-dependent pattern.
Bacterial Adaptation to Radiofrequency Electromagnetic Fields Based on Experiences from Ionizing Radiation
This 2025 review summarizes historical and modern literature on how bacteria may adapt to radiofrequency electromagnetic fields from common sources such as mobile phones and Wi-Fi. It argues that RF-EMF exposure can influence bacterial survival mechanisms and could potentially compromise therapeutic interventions by promoting increased resistance. The authors frame these possibilities as a public health concern and call for continued research and precaution.
Extremely Low-Frequency Magnetic Fields (ELF-MF) and Radiofrequency: Risk of Childhood CNS Tumors in a City with Elevated ELF-MF Exposure
This case-control study in Mexico City (2017–2022) evaluated residential ELF-MF and device-use proxies for RF exposure in relation to childhood CNS tumor risk. Elevated residential ELF-MF (≥0.4 μT) was associated with approximately doubled odds of CNST, while cell phone use showed no association. Prolonged tablet use, with or without internet connectivity, was reported to be associated with higher CNST risk.
Numerical analysis of the thermal effects on adult with brain pacemaker implantation exposed to WIFI antennas
This numerical study modeled RF exposure from WiFi/5G-type antennas near a 3D brain model with implanted brain pacemakers relevant to Parkinson’s disease. SAR and temperature increases were reported to remain below ICNIRP 2020 limits across modeled conditions, with maxima at a 90° antenna-to-brain angle. Despite compliance with SAR/temperature limits, the authors report modeled thermal strain and tissue displacement that could affect postoperative efficacy, leading them to recommend caution and increased distance from phones.
Simultaneous 4G and 5G EMF Exposure and Field Uniformity in a Reverberation Chamber for Animal Studies
This engineering study describes the design and validation of a reverberation chamber intended for large-scale animal carcinogenicity research with RF EMF relevant to 4G/5G. E-field uniformity was tested under four loading scenarios, including setups with 80 Sprague-Dawley rats. The chamber achieved better than 1.36 dB E-field uniformity across scenarios, and the authors report a method to predict composite E-field intensity for simultaneous multi-frequency exposures.
Effect of electromagnetic radiations from mobile towers on genetic damage and genetic polymorphism in humans: a review on India's perspective
This narrative review examines research on radiofrequency electromagnetic radiation (RF-EMR) from mobile towers and its potential association with genetic damage and genetic polymorphism in humans, with an emphasis on India. The abstract states that RF-EMR exposure may affect genetic material and suggests a link between EMR exposure and genetic damage, with possible implications for cancer risk and cell death. It also highlights that genetic polymorphisms may modify susceptibility and calls for further research to clarify health impacts.
Systematic reviews and meta-analyses for the WHO assessment of health effects of exposure to radiofrequency electromagnetic fields, an introduction
This editorial introduces a special issue supporting the WHO assessment of health effects from RF-EMF exposure, based on nine protocols and twelve systematic reviews developed over four years by more than 80 experts. It summarizes that human evidence for major cancers was moderate-certainty for no or only small effects, with lower certainty for some cancer sites, while animal evidence reported higher-certainty effects for several cancer types and adverse effects on male fertility. For cognition, symptoms, and oxidative stress, certainty was generally lower and findings more variable, and the editors note ongoing methodological challenges and the possibility of unidentified mechanisms.
Carcinogenicity of Radio-Frequency Radiation: Similarities and Differences Between Outcomes of Two Studies
This review compares two epidemiologic studies of radio-frequency radiation (RFR) exposure among military personnel with overlapping patient groups. One study reported a statistically significant increase in cancer among exposed individuals, while the other did not, which the review attributes to a smaller sample size. The review highlights similar cancer patterns across both studies, including a high proportion of hematolymphoid cancers and earlier onset among exposed individuals, and interprets these similarities as evidence of carcinogenic effects.
Altered development in rodent brain cells after 900 MHz radiofrequency exposure
This animal and in vitro study examined non-thermal 900 MHz RF-EMF exposure during prenatal and postnatal development at 0.08 and 0.4 W/kg SAR. The authors report changes consistent with altered neurodevelopment, including reduced BDNF, reduced in vivo cell proliferation, and disrupted synaptic balance in rat pup brain regions. In vitro, exposed neural stem cells showed increased apoptosis and DNA double-strand breaks and shifts in cell populations toward glial lineages. The authors conclude that regulatory-level 900 MHz exposure can disrupt key neurodevelopmental processes in rodents.
Looking for Biomarkers Which May Explain Idiopathic Environmental Intolerance Attributed to EMF (IEI-EMF): Does RF-EMF Exposure Influence Salivary Cortisol Response?
This randomized, double-blind, counterbalanced provocation study tested whether short-term RF-EMF exposure alters salivary cortisol in 72 predominantly young, healthy adults. Active exposure (2 W/kg peak SAR10g in head) showed no significant effect on cortisol compared with sham, and no sex differences were observed. The authors note that results may not rule out effects in populations not studied and encourage further research into plausible biological interactions.
Navigating Environmental Crossroads: Pesticides, Bee Pollinators, and the Wireless Revolution
This article summarizes a webinar series and frames pesticides and wireless radiation as concurrent environmental health crises affecting ecosystems and public health. It asserts that evidence is building for adverse effects of EMF/wireless radiation in humans, animals, and bees, including “high-certainty links” between RF radiation and tumors in brain and heart nerves. It also suggests potential synergy between chemical and EMF exposures impacting bee hive productivity and argues for precautionary policy and stronger exposure guidelines.
Impact of in vitro exposure to 5G-modulated 3.5 GHz fields on oxidative stress and DNA repair in skin cells
This in vitro study tested whether 5G-modulated 3.5 GHz RF-EMF exposure affects oxidative stress and DNA repair in human skin cells. Under acute exposure conditions (up to 24–48h) at SARs up to 4 W/kg, the authors report no significant changes in ROS markers, no adaptive response to oxidative challenge, and no impairment of UV-B–related CPD repair via nucleotide excision repair. The authors note that acute in vitro results may not directly generalize to chronic or real-life exposures.