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Changes in viability of rat adipose-derived stem cells isolated from abdominal/perinuclear adipose tissue stimulated with pulsed electromagnetic field.

Research RF Safe Research Library Jan 1, 2017

This in vitro study exposed rat adipose-derived stem cells to a pulsed electromagnetic field (7 Hz, 30 mT) and assessed viability by flow cytometry. The authors report that a low-fat diet was protective in PEMF-exposed ADSCs, especially in pups. They also report a higher proportion of early apoptotic cells in…

Cytotoxicity of temozolomide on human glioblastoma cells is enhanced by the concomitant exposure to an extremely low-frequency electromagnetic field (100Hz, 100G).

Research RF Safe Research Library Jan 1, 2017

This in vitro study tested whether an extremely low-frequency EMF (100 Hz, 100 G) could enhance temozolomide cytotoxicity in human glioma cell lines (U87 and T98G). The authors report that combined EMF and temozolomide increased apoptosis relative to either treatment alone and was accompanied by changes in…

Vitamin C and Vitamin E Protected B and Balb/c-3T3 Cells from Apoptosis Induced by Intermittent 50Hz ELF-EMF Radiation.

Research RF Safe Research Library Jan 1, 2017

This in vitro study exposed B cells and Balb/c-3T3 cells to intermittent 50 Hz power-line ELF-EMF and assessed apoptosis. The authors report that preincubation with vitamin C or vitamin E reduced apoptosis compared with the exposed group. They conclude that vitamins C and E have protective effects, with stronger…

Precision knockdown of EGFR gene expression using radio frequency electromagnetic energy.

Research RF Safe Research Library Jan 1, 2017

This study reports a technology that records a specific radiofrequency electromagnetic energy signal associated with EGFR siRNA and applies it to cells. The abstract describes reductions in EGFR mRNA and protein expression versus controls, with specificity assessed using RNA microarray and antibody dot blot array. It…

Enhancement of osteogenic differentiation of rat adipose tissue-derived mesenchymal stem cells by zinc sulphate under electromagnetic field via the PKA, ERK1/2 and Wnt/β-catenin signaling pathways.

Research RF Safe Research Library Jan 1, 2017

This in vitro study examined rat adipose-derived mesenchymal stem cells exposed to zinc sulphate in the presence of an ELF electromagnetic field (50 Hz, 20 mT). The authors report increased osteogenic differentiation markers (gene expression, ALP activity, and calcium levels) under combined ZnSO4+EMF conditions,…

Semi-quantitative proteomics of mammalian cells upon short-term exposure to non-ionizing electromagnetic fields.

Research RF Safe Research Library Jan 1, 2017

This in vitro study used proteome-wide semi-quantitative mass spectrometry to assess molecular changes after short-term exposure to ELF (50 Hz), UMTS (2.1 GHz), and WiFi (5.8 GHz) in human and mouse cell types. The authors report that fewer than 1% of quantified proteins changed slightly in abundance and that…

Efficacy of 50 Hz electromagnetic fields on human epidermal stem cell transplantation seeded in collagen sponge scaffolds for wound healing in a murine model.

Research RF Safe Research Library Jan 1, 2017

This animal study evaluated whether 50 Hz EMF exposure enhances wound healing after transplantation of human epidermal stem cells (ESC) seeded in collagen sponge scaffolds. Thirty-six nude mice with full-thickness wounds were assigned to control, ESC-only, or ESC+EMF groups (50 Hz, 5 mT, 60 min/day for 20 days). The…

Calreticulin attenuated microwave radiation-induced human microvascular endothelial cell injury through promoting actin acetylation and polymerization.

Research RF Safe Research Library Jan 1, 2017

This in vitro study exposed human microvascular endothelial cells to high-power microwave radiation at multiple power densities for 6 minutes and assessed cellular injury and cytoskeletal outcomes. The authors report dose-dependent injury from microwave exposure, including increased LDH leakage and reduced viability…

Gold Nanocluster-Mediated Cellular Death under Electromagnetic Radiation.

Research RF Safe Research Library Jan 1, 2017

This study investigated 2 nm protein-stabilized gold nanoclusters in six mammalian cell types under 1 GHz electromagnetic field exposure. The authors report low intrinsic toxicity of the nanoclusters, but a significant decrease in cell viability when nanocluster-incubated cells were exposed to the 1 GHz field,…

[Protective effects of Genistein on human renal tubular epithelial cells damage of microwave radiation].

Research RF Safe Research Library Jan 1, 2017

This in vitro study exposed human proximal renal tubular epithelial cells (HKC) to microwave irradiation for 24 hours and assessed cell injury, oxidative stress, and cell death. The authors report increased injury markers (LDH, NAG), increased oxidative stress (higher MDA, lower SOD), and more apoptosis/necrosis…

Fundamental study on a gene transfection methodology for mammalian cells using water-in-oil droplet deformation in a DC electric field.

Research RF Safe Research Library Jan 1, 2016

This paper describes an in vitro gene transfection technique termed water-in-oil droplet electroporation, using an intense DC electric field to deform a droplet between electrodes. The deformation can cause an instantaneous short circuit, during which transient membrane pores are reported to form and permit exogenous…

Targeted Magnetic Nanoparticles for Mechanical Lysis of Tumor Cells by Low-Amplitude Alternating Magnetic Field.

Research RF Safe Research Library Jan 1, 2016

This animal and in vitro study reports a method to lyse tumor cells mechanically using targeted superparamagnetic iron oxide nanoparticles rotated by a weak alternating magnetic field. Nude mice with head and neck tumors received cetuximab-coated nanoparticles and 4 Hz field exposures for 30 minutes on alternating…

Effects of extremely low frequency electromagnetic fields on in-vitro cellular cultures HeLa and CHO.

Research RF Safe Research Library Jan 1, 2016

This in-vitro study examined whether 50 Hz extremely low frequency electromagnetic fields affect proliferation in HeLa and CHO cell cultures. The abstract reports increased proliferation in exposed cells versus controls, with the largest reported increase under repetitive exposure at 2.49 mT (40 minutes every 12…

Disordered redox metabolism of brain cells in rats exposed to low doses of ionizing radiation or UHF electromagnetic radiation.

Research RF Safe Research Library Jan 1, 2016

This animal study assessed redox-state changes in rat brain cells after long-term low-intensity ionizing radiation exposure or fractionated non-thermal UHF electromagnetic radiation. Both exposures were associated with decreased ubisemiquinone and increased NO-FeS-protein complexes, alongside increased mitochondrial…

Magnetophoretic Conductors and Diodes in a 3D Magnetic Field.

Research RF Safe Research Library Jan 1, 2016

This engineering study demonstrates magnetophoretic track designs that transport single magnetized beads and magnetically labeled single cells in a 3D time-varying magnetic field. A vertical field bias is reported to reduce particle clustering but necessitates redesigned track geometries with alternating curvature.…

Effects of Wi-Fi (2.45 GHz) Exposure on Apoptosis, Sperm Parameters and Testicular Histomorphometry in Rats: A Time Course Study

Research RF Safe Research Library Jan 1, 2015

This animal experimental study exposed male Wistar rats to 2.45 GHz Wi-Fi radiation for 1 or 7 hours per day over two months and compared them with unexposed controls. The abstract reports time-dependent decreases in sperm parameters alongside increased apoptotic markers (apoptosis-positive cells and caspase-3…

Data supporting the role of electric field and electrode material on the improvement of the ageing effects in hydrogenated amorphous silicon solar cells.

Research RF Safe Research Library Jan 1, 2015

This Data in Brief article provides additional experimental data supporting a related study on mitigating ageing effects (Staebler-Wronski effect) in hydrogenated amorphous silicon solar cells. The associated method involves applying reverse bias stress and evaluating how temperature, electric field intensity,…

[Synergistic inhibitory effect of static magnetic field and antitumor drugs on Hepa1-6 cells].

Research RF Safe Research Library Jan 1, 2015

This in vitro study tested whether a static magnetic field (SMF) could enhance the effects of cisplatin or adriamycin in Hepa1-6 tumor cells. The abstract reports that SMF combined with either drug increased growth inhibition and was associated with cell-cycle arrest, increased DNA damage, and more severe membrane…

Electromagnetic fields counteract IL-1β activity during chondrogenesis of bovine mesenchymal stem cells.

Research RF Safe Research Library Jan 1, 2015

This in vitro study examined whether electromagnetic fields (EMFs) influence chondrogenic differentiation of bovine mesenchymal stem cell pellets, with and without the inflammatory cytokine IL-1β. The authors report EMFs had limited effects on chondrogenesis in the absence of inflammation, but in the presence of…

Electromagnetic fields (UHF) increase voltage sensitivity of membrane ion channels; possible indication of cell phone effect on living cells.

Research RF Safe Research Library Jan 1, 2015

This in vitro study used voltage clamp recordings of single OmpF porin channels in an artificial bilayer to test effects of UHF EMF (910–990 MHz). The authors report frequency-dependent changes in channel gating, with increased gating frequency and voltage sensitivity and a maximum effect at 930 MHz. Conductance was…

Experimental model for ELF-EMF exposure: Concern for human health.

Research RF Safe Research Library Jan 1, 2015

This in vitro study examined whether extremely low frequency EMF exposure alters MCP-1 expression in several human cell lines. Cells were exposed to a 50 Hz sinusoidal field at 1 mT (rms), and the authors report that MCP-1 expression was modified in a cell-type-dependent manner. The findings are described as…

Effects of a continuous electromagnetic field on wound healing in human airway.

Research RF Safe Research Library Jan 1, 2015

This in vitro study exposed BEAS-2B human bronchial epithelial cells to a 1.8-GHz continuous EMF at SAR 1.0 W/kg and assessed wound-healing related endpoints. EMF exposure decreased cell migration in a scratch assay compared with controls. The authors report no cytotoxicity, no inhibition of proliferation, and no…

Melatonin protects rat cerebellar granule cells against electromagnetic field-induced increases in Na(+) currents through intracellular Ca(2+) release

Research RF Safe Research Library Jan 1, 2014

This in vitro study examined extremely low-frequency electromagnetic field (ELF-EMF) effects on voltage-gated sodium (Nav) currents in rat cerebellar granule cells and whether melatonin modifies these effects. The abstract reports that 60 minutes of ELF-EMF exposure increased Nav current density, and that melatonin…

Assessment of the neurotoxic potential of exposure to 50Hz extremely low frequency electromagnetic fields (ELF-EMF) in naïve and chemically stressed PC12 cells

Research RF Safe Research Library Jan 1, 2014

This in vitro study tested acute (30 min) and sub-chronic (48 h) 50 Hz ELF-EMF exposure up to 1000 µT in naïve and chemically stressed PC12 cells. Across assays of Ca2+ homeostasis, ROS production, and membrane integrity, the study reports no consistent or measurable neurotoxic effects under the tested conditions.…

Effect of ELF-EMF Exposure on Human Neuroblastoma Cell Line: a Proteomics Analysis

Research RF Safe Research Library Jan 1, 2014

This in vitro study exposed SH-SY5Y human neuroblastoma cells to a 2 mT, 50 Hz magnetic field for 3 hours and assessed protein expression using proteomics. The authors report that 189 proteins changed expression in exposed cells compared with controls, with PCA/clustering separating samples by condition. They…

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