Fabrication and physicochemical characterization of a novel magnetic nanocomposite scaffold: Electromagnetic field effect on biological properties.
Research
RF Safe Research Library
Jan 1, 2020
This in vitro study fabricated PCL nanofibrous scaffolds containing cobalt-zinc ferrite nanoparticles and evaluated physicochemical/mechanical properties and cell-related outcomes. The abstract reports improved hydrophilicity, biodegradation, and stress at break with CZF-NP incorporation, and improved L929 cell…
Electromagnetic field affects the voltage-dependent potassium channel Kv1.3.
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RF Safe Research Library
Jan 1, 2020
This in vitro study tested whether extremely low-frequency electromagnetic fields (ELF-EMF) modulate the voltage-gated potassium channel Kv1.3 in Kv1.3-expressing CHO-K1 cells. Cells were exposed to a 20 Hz field at 268 µT or 902 µT while Kv1.3 currents were measured by whole-cell patch clamp. The authors report a…
Effects of pulse-modulated radiofrequency magnetic field (RF-EMF) exposure on apoptosis, autophagy, oxidative stress and electron chain transport function in human neuroblastoma and murine microglial cells.
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Jan 1, 2020
This in vitro study exposed murine microglial (N9) and human neuroblastoma (SH-SY5Y) cells to ELF-modulated 935 MHz RF-EMF at 4 W/kg for 2 or 24 hours. The authors report no changes in apoptosis, cell viability, or AIF across conditions. They report increased ATG5 after 24 hours and a transient glutathione increase…
Characterizing the Binding of Angiotensin Converting Enzyme I Inhibitory Peptide to Human Hemoglobin: Influence of Electromagnetic Fields.
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RF Safe Research Library
Jan 1, 2020
This in vitro study used spectroscopic and conductometry methods to examine binding between human hemoglobin and an angiotensin-converting enzyme inhibitory peptide, with and without electromagnetic field (EMF) exposure. The authors report that EMF changed the Hb–ACEIP interaction and that binding was associated with…
The effect of electromagnetic fields on survival and proliferation rate of dental pulp stem cells.
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RF Safe Research Library
Jan 1, 2020
This in vitro study tested whether 50 Hz extremely low-frequency electromagnetic fields (0.5 or 1 mT) affect human dental pulp stem cell survival and proliferation. Using MTT and DAPI assays after 7 days of exposure (20 or 40 min/day), all exposed groups reportedly showed higher viability/proliferation than controls.…
Exploring the form- And time-dependent effect of low-frequency electromagnetic fields on maintenance of hippocampal long-term potentiation.
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Jan 1, 2020
This in vitro study developed an MEA-compatible magnetic stimulation device to deliver low-frequency EMFs during hippocampal LTP experiments without interrupting induction. The authors report that sine-wave LF-EMFs applied post-induction or as priming reduced fEPSP slope during LTP maintenance, with stronger effects…
Osteoinductivity of polycaprolactone nanofibers grafted functionalized with carboxymethyl chitosan: Synergic effect of β-carotene and electromagnetic field.
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RF Safe Research Library
Jan 1, 2020
This in vitro study developed carboxymethyl chitosan (CMC)-grafted polycaprolactone (PCL) nanofiber scaffolds for bone tissue engineering. It reports mineralization and osteonectin expression in adipose mesenchymal stem cells cultured on the scaffolds, suggesting osteoinductive properties of the grafted CMC. The…
Extremely Low-Frequency Electromagnetic Fields Increase the Expression of Anagen-Related Molecules in Human Dermal Papilla Cells via GSK-3β/ERK/Akt Signaling Pathway.
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RF Safe Research Library
Jan 1, 2020
This in vitro study exposed human dermal papilla cells to 70 Hz extremely low-frequency electromagnetic fields at 5–100 G for four days. The abstract reports increased expression of several anagen-related molecules and increased proliferation, with 10 G described as the most potent condition. It also reports…
A single-pulsed electromagnetic field enhances collagen synthesis in tendon cells.
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RF Safe Research Library
Jan 1, 2020
This in vitro study reports that a single pulsed electromagnetic field (0.2 Hz) increased expression of tenogenic genes and reduced expression of a matrix-degradation/inflammatory gene (Mmp1) in tendon cells. After 5 days of exposure (3 min/day), collagen type I and total collagen synthesis protein levels were…
Impact of electromagnetic fields on in vitro toxicity of silver and graphene nanoparticles.
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RF Safe Research Library
Jan 1, 2019
This in vitro study examined whether RF electromagnetic fields (900, 2400, 7500 MHz) modify the toxicity of silver and graphene nanoparticles in human fibroblast cultures. The authors report EMF-associated changes in cytotoxicity and ROS that depended on nanoparticle type, shape, and concentration, with the strongest…
The combinatory effect of sinusoidal electromagnetic field and VEGF promotes osteogenesis and angiogenesis of mesenchymal stem cell-laden PCL/HA implants in a rat subcritical cranial defect.
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RF Safe Research Library
Jan 1, 2019
This animal and in vitro study evaluated sinusoidal electromagnetic field (SEMF) exposure combined with VEGF in rat mesenchymal stem cells and in a rat cranial defect model using rBMSC-laden PCL/HA scaffolds. The authors report increased osteogenic and endothelial marker expression with SEMF and VEGF, with a slight…
T-Cell Differentiation to T Helper 9 Phenotype is Elevated by Extremely Low-Frequency Electromagnetic Fields Via Induction of IL-2 Signaling.
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RF Safe Research Library
Jan 1, 2019
This in vitro study examined 60 Hz extremely low-frequency EMF exposure (0.8 mT) on CD4 T cells under Th9-polarizing conditions. The authors report increased Th9-associated responses including IL-9 secretion, transcription factor changes (including early GATA3), and increased STAT5/STAT6 phosphorylation alongside…
Impact of extremely low-frequency electromagnetic field (100 Hz, 100 G) exposure on human glioblastoma U87 cells during Temozolomide administration.
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RF Safe Research Library
Jan 1, 2019
This in vitro study evaluated whether extremely low-frequency EMF exposure (100 Hz, 100 G) modifies the effects of temozolomide (100 µM) in human glioblastoma U87 cells over 120–144 hours. The authors report that combined treatment reduced stemness-associated markers (Nestin, CD133, Notch4) and increased GFAP…
Efficacy of pulsed electromagnetic fields and electromagnetic fields tuned to the ion cyclotron resonance frequency of Ca on chondrogenic differentiation.
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RF Safe Research Library
Jan 1, 2019
This in vitro study compared commonly used pulsed electromagnetic field (PEMF) exposures with electromagnetic fields tuned to satisfy the ion cyclotron resonance (ICR) condition for calcium. After 3 weeks, the calcium ICR condition was associated with increased glycosaminoglycans and type II collagen synthesis and…
Effects of single and combined low frequency electromagnetic fields and simulated microgravity on gene expression of human mesenchymal stem cells during chondrogenesis.
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RF Safe Research Library
Jan 1, 2018
This in vitro study exposed human mesenchymal stem cell pellets undergoing chondrogenesis to simulated microgravity (SMG), low-frequency EMF (5 mT, 15 Hz), or both for 3 weeks. SMG significantly changed gene expression and reduced chondrogenic marker expression (including COL2A1). LF-EMF alone showed no…
Evaluation of effect of high frequency electromagnetic field on growth and antibiotic sensitivity of bacteria.
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RF Safe Research Library
Jan 1, 2018
This in vitro study evaluated whether high-frequency electromagnetic fields at 900 and 1800 MHz affect bacterial DNA, growth, and antibiotic susceptibility after 2 hours of exposure. The authors report no detectable DNA differences between exposed and non-exposed bacteria for the species described. Growth and…
Acute exposure to high-induction electromagnetic field affects activity of model peripheral sensory neurons.
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Jan 1, 2018
This in vitro study examined acute low-frequency electromagnetic field (LF-EMF) exposure using high-induction magnetic stimulation in F11 cells, a dorsal root ganglia neuron-derived model of peripheral sensory neurons. Short-term exposure (
Synergistic effect of rice bran extract and extremely low-frequency electromagnetic fields on dermal papilla/melanocytes in melanogenesis.
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RF Safe Research Library
Jan 1, 2018
This in vitro study used a 3D dermal papilla-like tissue model containing dermal papilla cells and melanocytes to assess melanogenesis after rice bran extract (RBE), extremely low-frequency EMF, or their combination. The combined RBE/EMF condition showed higher tyrosinase activity, increased melanogenesis-related…
Fas/FasL pathway and cytokines in keratinocytes in atopic dermatitis - Manipulation by the electromagnetic field.
Research
RF Safe Research Library
Jan 1, 2018
This in vitro study exposed keratinocytes from atopic dermatitis patients and healthy controls, plus two keratinocyte cell lines, to a 900 MHz electromagnetic field for 60 minutes. AD-derived keratinocytes showed higher Fas/FasL signaling markers and increased secretion of multiple cytokines versus controls. EMF…
Non-Ionizing Radiation for Cardiac Human Amniotic Mesenchymal Stromal Cell Commitment: A Physical Strategy in Regenerative Medicine.
Research
RF Safe Research Library
Jan 1, 2018
This in vitro study exposed human amniotic mesenchymal stromal cells to an ELF-EMF stimulus (7 Hz, 2.5 µT) and compared responses with a nitric oxide chemical treatment. The authors report reduced cell proliferation with no change in metabolic activity, vitality, or apoptosis after ELF-EMF exposure. They also report…
A 60 Hz uniform electromagnetic field promotes human cell proliferation by decreasing intracellular reactive oxygen species levels.
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RF Safe Research Library
Jan 1, 2018
This in vitro study examined effects of a 6 mT, 60 Hz uniform ELF electromagnetic field on HeLa cells and primary IMR-90 fibroblasts. Single or repetitive exposure did not induce DNA damage or change viability, but continuous exposure over 168 hours was reported to increase proliferation in both cell types in a…
Effects of electromagnetic field, cisplatin and morphine on cytotoxicity and expression levels of DNA repair genes.
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RF Safe Research Library
Jan 1, 2018
This in vitro study examined how morphine, a 50-Hz 0.50 mT electromagnetic field (intermittent), and cisplatin co-treatments affected DNA repair gene expression and cytotoxicity in MCF-7 and SH-SY5Y cells. Morphine alone was reported to down-regulate examined DNA repair genes, whereas morphine plus EMF did not…
Low frequency electromagnetic field decreases ischemia-reperfusion injury of human cardiomyocytes and supports their metabolic function.
Research
RF Safe Research Library
Jan 1, 2018
This in vitro study tested whether extremely low frequency/low frequency electromagnetic field (LF-EMF) exposure modifies ischemia-reperfusion injury in human cardiac-related cell types. Using LDH and MMP-2 activities and fluorescein diacetate staining, the authors report that short courses of LF-EMF reduced cellular…
Evaluation of Cytotoxic and Genotoxic Effects of Extremely Low-frequency Electromagnetic Field on Mesenchymal Stromal Cells.
Research
RF Safe Research Library
Jan 1, 2018
This in vitro study evaluated whether a therapeutic extremely low-frequency EMF exposure (5 Hz, 0.4 mT) causes cytotoxic or genotoxic effects in a human mesenchymal stromal cell line. Cells were exposed for 20 minutes per day, three times per week, for two weeks and assessed for viability, proliferation, and…