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Effects of extremely low frequency electromagnetic fields on in-vitro cellular cultures HeLa and CHO.

Research RF Safe Research Library Jan 1, 2016

This in-vitro study examined whether 50 Hz extremely low frequency electromagnetic fields affect proliferation in HeLa and CHO cell cultures. The abstract reports increased proliferation in exposed cells versus controls, with the largest reported increase under repetitive exposure at 2.49 mT (40 minutes every 12…

[Genic and non-genic regulation of low frequence pulsed electromagnetic fields on osteoblasts differentiation].

Research RF Safe Research Library Jan 1, 2016

This in vitro study exposed rat calvarial osteoblasts to 50 Hz, 0.6 mT pulsed electromagnetic fields for 1.5 hours and measured early differentiation-related markers over 0–12 hours. The abstract reports increased ALP activity at 3 hours and time-dependent changes in ALP, Runx2, and OSX transcription and Runx2/OSX…

[Effects of 1.8 mT sinusoidal alternating electromagnetic fields of different frequencies on bone biomechanics of young rats].

Research RF Safe Research Library Jan 1, 2016

This randomized animal experiment exposed 32 young female rats to 1.8 mT sinusoidal alternating electromagnetic fields at 10, 25, or 40 Hz for 90 minutes per day. The 10 Hz and 40 Hz groups had higher BMD and improved femur and lumbar vertebra biomechanical properties compared with controls, and micro-CT suggested…

Effect of exposure to extremely low frequency magnetic fields on melatonin levels in calves is seasonally dependent

Research RF Safe Research Library Sep 18, 2015

This animal study measured salivary melatonin in 1–2 month old calves exposed to 50 Hz extremely low frequency magnetic fields in the hundreds of nT range. The authors report a season-dependent response: melatonin decreased with exposure in winter but increased in summer. They suggest future studies should consider…

Low-Frequency Electromagnetic Field Exposure Enhances Extracellular Trap Formation by Human Neutrophils through the NADPH Pathway

Research RF Safe Research Library Jan 1, 2015

This in vitro/ex vivo study examined whether low-frequency electromagnetic field exposure affects human neutrophil extracellular trap (NET) formation. The authors report that LF EMF exposure enhanced NET formation and that this effect depended on the NADPH oxidase pathway and reactive oxygen species production. They…

[Influence of magnetic field on the growth of pathogen microorganisms isolated from the indoor environment at the Archivo Nacional de la República de Cuba].

Research RF Safe Research Library Jan 1, 2015

This in vitro study exposed five microorganisms (including indoor isolates and reference strains) to an extremely low frequency magnetic field (60 Hz, 3 mT) for two hours. The authors report a statistically significant increase in colony counts in exposed samples versus controls, with stronger stimulation in nutrient…

[Influence of Low-Frequency Electromagnetic Field on DNA Molecules in Water Solutions].

Research RF Safe Research Library Jan 1, 2015

This in vitro study reports that hepatitis B and C virus DNA amplicons in water solutions produced a chemiluminescence-registered signal after exposure to low-frequency electromagnetic fields (3–30 Hz; 24–40 A/m). The strongest reported effect occurred at 9 Hz, alongside an observed change in the DNA hydration shell.…

The use of low-level electromagnetic fields to suppress atrial fibrillation.

Research RF Safe Research Library Jan 1, 2015

This animal study tested whether extremely low-level pulsed EMF (0.034 μG, 0.952 Hz) could suppress induced atrial fibrillation in 17 anesthetized dogs. EMF applied to cervical vagal trunks or across the chest was reported to suppress neural firing in the anterior right ganglionated plexi. The authors report that EMF…

Effects of extremely low-frequency electric fields at different intensities and exposure durations on mismatch negativity

Research RF Safe Research Library Jul 11, 2014

This rat study examined whether 50-Hz extremely low-frequency electric fields at 12 or 18 kV/m for 2 or 4 weeks affect mismatch negativity (MMN) and markers of oxidative damage/apoptosis in brain tissue. MMN amplitude was reported to be significantly reduced only in the 18 kV/m, 4-week exposure group versus the…

Sleep quality and general health status of employees exposed to extremely low frequency magnetic fields in a petrochemical complex

Research RF Safe Research Library Apr 29, 2014

This cross-sectional study measured ELF magnetic flux density in a petrochemical complex substation environment and assessed workers' sleep quality (PSQI) and general health (GHQ). It reports significant differences between case and control groups, with more sleep disorder and poorer health status in the case group.…

Melatonin protects rat cerebellar granule cells against electromagnetic field-induced increases in Na(+) currents through intracellular Ca(2+) release

Research RF Safe Research Library Jan 1, 2014

This in vitro study examined extremely low-frequency electromagnetic field (ELF-EMF) effects on voltage-gated sodium (Nav) currents in rat cerebellar granule cells and whether melatonin modifies these effects. The abstract reports that 60 minutes of ELF-EMF exposure increased Nav current density, and that melatonin…

Occupational exposure to intermediate frequency and extremely low frequency magnetic fields among personnel working near electronic article surveillance systems

Research RF Safe Research Library Jan 1, 2014

This workplace measurement study assessed intermediate frequency (IF) and extremely low frequency (ELF) magnetic field exposure among cashiers working near electronic article surveillance (EAS) systems in Finnish stores. Typical seated exposures were reported as well below limits, but short-term exceedances of ICNIRP…

Effect of ELF-EMF Exposure on Human Neuroblastoma Cell Line: a Proteomics Analysis

Research RF Safe Research Library Jan 1, 2014

This in vitro study exposed SH-SY5Y human neuroblastoma cells to a 2 mT, 50 Hz magnetic field for 3 hours and assessed protein expression using proteomics. The authors report that 189 proteins changed expression in exposed cells compared with controls, with PCA/clustering separating samples by condition. They…

The effect of extremely low frequency pulsed electromagnetic field on in vitro fertilization success rate in N MRI mice.

Research RF Safe Research Library Jan 1, 2014

This randomized comparative animal study tested whether extremely low frequency pulsed electromagnetic field exposure affects IVF success in NMRI mice. Mice in the experimental group were exposed to a 1.3 mT pulsed field at 4 kHz for 5 hours during the IVF process. Although the exposed group showed a higher IVF…

Assessment of extremely low frequency magnetic field exposure from GSM mobile phones.

Research RF Safe Research Library Jan 1, 2014

This paper reports extremely low frequency (ELF) magnetic flux density measurements from 47 GSM mobile phones, collected to support exposure classification in the MOBI-KIDS epidemiological study. At 25 mm, maximum resultant magnetic flux density at 217 Hz had a geometric mean of 221 nT, and inclusion of harmonics…

Electromagnetic fields act via activation of voltage-gated calcium channels to produce beneficial or adverse effects

Research RF Safe Research Library Jan 1, 2013

This narrative review argues that non-thermal biological effects of extremely low and microwave frequency EMFs may be mediated by activation of voltage-gated calcium channels (VGCCs). It cites 23 studies in which VGCC blockers reportedly block or reduce diverse EMF effects and proposes downstream…

Influence of pulsed magnetic field on soybean (Glycine max L.) seed germination, seedling growth and soil microbial population.

Research RF Safe Research Library Jan 1, 2013

This laboratory study examined pulsed magnetic field (PMF) pretreatment of soybean seeds (1500 nT) at 0.1–100 Hz for 5 hours/day over 20 days. The abstract reports increased germination rates for all PMF treatments, with 10 and 100 Hz showing the strongest responses, and improvements in several seedling growth and…

P-selectin expression in a colon tumor model exposed by sinusoidal electromagnetic fields.

Research RF Safe Research Library Jan 1, 2013

This animal study evaluated whether sinusoidal magnetic field exposure alters P-selectin expression in a rat colon tumor model. Rats exposed to 50 Hz, 5 mT for 6 h/day for 8 months showed no statistically significant change in P-selectin expression by immunohistochemistry. The authors indicate that further in vitro…

Effect of extremely low frequency electromagnetic field exposure on sleep quality in high voltage substations.

Research RF Safe Research Library Jan 1, 2012

This case-control study measured electric field intensity and magnetic flux density in high voltage substations and estimated workers' ELF-EMF exposure over a shift. Sleep quality was assessed using the PSQI in 67 exposed workers and 110 controls. The study reports no statistically significant difference in overall…

Response identification in the extremely low frequency region of an electret condenser microphone.

Research RF Safe Research Library Jan 1, 2011

This engineering study describes an experimental setup using a small electret condenser microphone connected to a notebook/PC to detect extremely low frequency signals. Using a small air cell made from a drilled DVD plate with a speaker and microphone on opposite sides, the microphone captured signals in the 0.5–20…

[Time effect of sinusoidal electromagnetic field on enhancing the maturation and mineralization of osteoblasts in vitro].

Research RF Safe Research Library Jan 1, 2011

This in vitro study exposed newborn rat calvarial osteoblasts to 50 Hz sinusoidal electromagnetic fields at 1.8 mT for different daily exposure times (0.5–3.0 h). The authors report inhibited osteoblast proliferation at longer exposure times, alongside increased differentiation markers (ALP activity) and…

Effects of low frequency electromagnetic fields on the chondrogenic differentiation of human mesenchymal stem cells.

Research RF Safe Research Library Jan 1, 2011

This in vitro study examined whether extremely low-frequency magnetic fields affect chondrogenic differentiation of human mesenchymal stem cells in pellet culture. Cells exposed for 3 weeks to a homogeneous sinusoidal 5 mT magnetic field showed increased collagen type II expression (significant at passage 6) and…

Ohmic heating of peaches in the wide range of frequencies (50 Hz to 1 MHz).

Research RF Safe Research Library Jan 1, 2010

This engineering/food science study examined ohmic heating of peaches at 60 V/cm using bipolar pulses across frequencies from 50 Hz to 1 MHz. Heating time to a target temperature and multiple electrical properties were frequency-dependent, particularly for intact tissue. The authors report faster electro-thermal…

[Effects of 50 Hz sinusoidal magnetic field on Ca2+ release channel ryanodine receptor of sarcoplasmic reticulum vesicles].

Research RF Safe Research Library Jan 1, 2009

This in vitro study examined whether a 50 Hz sinusoidal magnetic field affects the sarcoplasmic reticulum calcium release channel RyR1. A 0.4 mT exposure for 30 minutes increased SR Ca2+ release rate and [(3)H]-ryanodine binding under NADH/NAD+ conditions. The authors also report increased NADH oxidation and…

The effect of GSM-like ELF radiation on the alpha band of the human resting EEG.

Research RF Safe Research Library Jan 1, 2008

This double-blind, counterbalanced cross-over study investigated whether extremely low frequency (DC to at least 40 kHz) fields emitted by GSM handsets affect resting EEG. In 72 healthy volunteers, the authors report a decrease in the alpha band during 20 minutes of ELF exposure in the exposed hemisphere only. They…

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