Crosstalk between 6-methyladenine and 4-methylcytosine in exposed to extremely low-frequency electromagnetic field.
Research
RF Safe Research Library
Jan 1, 2024
This study examined prokaryotic DNA methylation (6mA and 4mC) and transcriptomes under different intensities of extremely low-frequency electromagnetic field (ELF-EMF) exposure. It reports motif-specific 6mA at 5'-GTACAG-3' and ELF-EMF-intensity-associated changes in spatial relationships among other 6mA sites,…
Exposure to the extremely low-frequency electromagnetic field induces changes in the epigenetic regulation of gene expression in the endometrium.
Research
RF Safe Research Library
Jan 1, 2024
This in vitro study exposed porcine endometrial slices from days 15–16 of pregnancy to a 50 Hz extremely low-frequency electromagnetic field for 2 hours. The authors report changes in several epigenetic-related markers, including DNA methylation machinery, histone-related measures (HDAC activity), global DNA…
NET Formation Was Reduced via Exposure to Extremely Low-Frequency Pulsed Electromagnetic Fields.
Research
RF Safe Research Library
Jan 1, 2023
This in vitro study examined whether a 16 Hz extremely low-frequency pulsed electromagnetic field (ELF-PEMF) affects neutrophil extracellular trap (NET) formation. In neutrophils from healthy volunteers, ELF-PEMF exposure did not induce ROS or calcium influx and did not increase basal or PMA-induced NET formation.…
Extremely low-frequency electromagnetic field exposure alters DNA methylation levels in the endometrium of pigs during the peri-implantation period.
Research
RF Safe Research Library
Jan 1, 2023
This in vitro study exposed porcine endometrial slices from the peri-implantation period (days 15–16 of pregnancy) to 50 Hz ELF-EMF for 2 hours and assessed promoter DNA methylation in selected genes. The authors report increased methylation for EGR2, ID2, and PTGER4, decreased methylation for IL1RAP and NOS3, and no…
Pulsed and Discontinuous Electromagnetic Field Exposure Decreases Temozolomide Resistance in Glioblastoma by Modulating the Expression of O-Methylguanine-DNA Methyltransferase, Cyclin-D1, and p53.
Research
RF Safe Research Library
Jan 1, 2021
This in vitro study evaluated 50 Hz, 70 G electromagnetic field exposure in combination with temozolomide (TMZ) in T98 and A172 glioblastoma cell lines. The authors report that EMF plus TMZ increased cell death and reduced migration potential compared with controls. They also report increased p53 gene expression and…
Exposure to long-term evolution radiofrequency electromagnetic fields decreases neuroblastoma cell proliferation via Akt/mTOR-mediated cellular senescence.
Research
RF Safe Research Library
Jan 1, 2021
This in vitro study examined LTE RF-EMF exposure effects on SH-SY5Y neuroblastoma cells. The authors report reduced cell proliferation and a G0/G1 cell-cycle delay after 1760 MHz exposure at 4 W/kg for 4 hr/day over 4 days. They report no marked DNA damage signal (γH2AX) and no apoptosis activation, but increased…
Exposure to mobile phone radiations at 2350 MHz incites cyto- and genotoxic effects in root meristems of .
Research
RF Safe Research Library
Jan 1, 2019
This experimental study exposed onion root meristems to continuous 2350 MHz radiofrequency EMF for 1, 2, or 4 hours and assessed cytotoxicity and genotoxicity. It reports increased mitotic index at 4 hours and increased chromosomal aberrations at exposures of 2 hours or longer, with some comet assay DNA distribution…
Evaluation of effect of high frequency electromagnetic field on growth and antibiotic sensitivity of bacteria.
Research
RF Safe Research Library
Jan 1, 2018
This in vitro study evaluated whether high-frequency electromagnetic fields at 900 and 1800 MHz affect bacterial DNA, growth, and antibiotic susceptibility after 2 hours of exposure. The authors report no detectable DNA differences between exposed and non-exposed bacteria for the species described. Growth and…
Impact of Long-Term RF-EMF on Oxidative Stress and Neuroinflammation in Aging Brains of C57BL/6 Mice.
Research
RF Safe Research Library
Jan 1, 2018
This animal study exposed 14-month-old C57BL/6 mice to 1950 MHz RF-EMF (SAR 5 W/kg) for 8 months. The authors report that aging was associated with increased oxidative damage, DNA damage, apoptosis, and glial activation markers in brain tissue. Long-term RF-EMF exposure did not change these markers in aged mice and…
A 60 Hz uniform electromagnetic field promotes human cell proliferation by decreasing intracellular reactive oxygen species levels.
Research
RF Safe Research Library
Jan 1, 2018
This in vitro study examined effects of a 6 mT, 60 Hz uniform ELF electromagnetic field on HeLa cells and primary IMR-90 fibroblasts. Single or repetitive exposure did not induce DNA damage or change viability, but continuous exposure over 168 hours was reported to increase proliferation in both cell types in a…
Effects of electromagnetic field, cisplatin and morphine on cytotoxicity and expression levels of DNA repair genes.
Research
RF Safe Research Library
Jan 1, 2018
This in vitro study examined how morphine, a 50-Hz 0.50 mT electromagnetic field (intermittent), and cisplatin co-treatments affected DNA repair gene expression and cytotoxicity in MCF-7 and SH-SY5Y cells. Morphine alone was reported to down-regulate examined DNA repair genes, whereas morphine plus EMF did not…
Effects of 1800 MHz RF-EMF exposure on DNA damage and cellular functions in primary cultured neurogenic cells.
Research
RF Safe Research Library
Jan 1, 2018
This in vitro study exposed primary cultured astrocytes, microglia, and cortical neurons to 1800 MHz RF-EMF at 4.0 W/kg SAR to assess DNA damage and cellular functions. The authors report no significant induction of γH2AX foci and no significant changes in cytokine secretion or dendrite/synapse morphological…
Genotoxic Effects in Human Fibroblasts Exposed to Microwave Radiation.
Research
RF Safe Research Library
Jan 1, 2018
This in vitro study exposed human fetal and adult fibroblasts to 25 GHz microwave radiation and assessed multiple genotoxic and cellular endpoints. The authors report no evidence of direct DNA damage and no apoptosis or changes in prosurvival signalling proteins. However, they report increased total and…
Oxidative and mutagenic effects of low intensity GSM 1800 MHz microwave radiation
Research
RF Safe Research Library
Jan 1, 2018
This animal study used developing quail embryos to assess oxidative and mutagenic effects of low-intensity GSM 1800 MHz radiation from a commercial smartphone. The authors report statistically significant increases in oxidative stress markers, DNA integrity damage, and oxidative DNA damage (8-oxo-dG) in exposed…
RF-EMF exposure at 1800 MHz did not elicit DNA damage or abnormal cellular behaviors in different neurogenic cells.
Research
RF Safe Research Library
Jan 1, 2017
This in vitro study assessed whether intermittent 1800 MHz RF-EMF exposure induces DNA damage or alters cellular behavior in three neurogenic cell lines. Cells were exposed at an average SAR of 4.0 W/kg for 1, 6, or 24 hours and evaluated for γH2AX foci, cell cycle progression, proliferation, and viability. The…
Different profiles of the mRNA levels of DNA repair genes in MCF-7 and SH-SY5Y cells after treatment with combination of cisplatin, 50-Hz electromagnetic field and bleomycin.
Research
RF Safe Research Library
Jan 1, 2017
This in vitro study examined whether a 50-Hz, 0.50 mT intermittent electromagnetic field modifies responses to cisplatin and bleomycin in MCF-7 and SH-SY5Y cells. The authors report increased susceptibility in MCF-7 cells with EMF added to cisplatin+bleomycin, while SH-SY5Y susceptibility was unchanged. They also…
Electromagnetic fields at a mobile phone frequency (900 MHz) trigger the onset of general stress response along with DNA modifications in Eisenia fetida earthworms.
Research
RF Safe Research Library
Jan 1, 2017
This animal study exposed Eisenia fetida earthworms to 900 MHz electromagnetic fields at 10–120 V/m for 2 hours (with additional tests of longer exposure, modulation, and recovery). The authors report significant DNA modifications across exposure treatments and a decrease in probe hybridisation sites persisting after…
Efficient DNA ligation by selective heating of DNA ligase with a radio frequency alternating magnetic field.
Research
RF Safe Research Library
Jan 1, 2016
This in vitro study describes a method to improve DNA ligation by immobilizing T4 DNA ligase on ferromagnetic particles and applying a radio frequency alternating magnetic field. The field causes the particles to dissipate heat, which the authors report selectively heats and activates the ligase while minimally…
Fundamental study on a gene transfection methodology for mammalian cells using water-in-oil droplet deformation in a DC electric field.
Research
RF Safe Research Library
Jan 1, 2016
This paper describes an in vitro gene transfection technique termed water-in-oil droplet electroporation, using an intense DC electric field to deform a droplet between electrodes. The deformation can cause an instantaneous short circuit, during which transient membrane pores are reported to form and permit exogenous…
[Synergistic inhibitory effect of static magnetic field and antitumor drugs on Hepa1-6 cells].
Research
RF Safe Research Library
Jan 1, 2015
This in vitro study tested whether a static magnetic field (SMF) could enhance the effects of cisplatin or adriamycin in Hepa1-6 tumor cells. The abstract reports that SMF combined with either drug increased growth inhibition and was associated with cell-cycle arrest, increased DNA damage, and more severe membrane…
[Influence of Low-Frequency Electromagnetic Field on DNA Molecules in Water Solutions].
Research
RF Safe Research Library
Jan 1, 2015
This in vitro study reports that hepatitis B and C virus DNA amplicons in water solutions produced a chemiluminescence-registered signal after exposure to low-frequency electromagnetic fields (3–30 Hz; 24–40 A/m). The strongest reported effect occurred at 9 Hz, alongside an observed change in the DNA hydration shell.…
Electromagnetic fields act via activation of voltage-gated calcium channels to produce beneficial or adverse effects
Research
RF Safe Research Library
Jan 1, 2013
This narrative review argues that non-thermal biological effects of extremely low and microwave frequency EMFs may be mediated by activation of voltage-gated calcium channels (VGCCs). It cites 23 studies in which VGCC blockers reportedly block or reduce diverse EMF effects and proposes downstream…
Effect of electromagnetic radiofrequency radiation on the rats' brain, liver and kidney cells measured by comet assay.
Research
RF Safe Research Library
Jan 1, 2011
This animal study assessed DNA damage in rat brain, liver, and kidney cells after repeated whole-body exposure to 915 MHz GSM-modulated RF radiation (SAR 0.6 W/kg) using the comet assay. Tail length was slightly increased in brain cells and differed more notably in liver and kidney samples versus sham controls, while…
Effects of low frequency electromagnetic fields on the chondrogenic differentiation of human mesenchymal stem cells.
Research
RF Safe Research Library
Jan 1, 2011
This in vitro study examined whether extremely low-frequency magnetic fields affect chondrogenic differentiation of human mesenchymal stem cells in pellet culture. Cells exposed for 3 weeks to a homogeneous sinusoidal 5 mT magnetic field showed increased collagen type II expression (significant at passage 6) and…
Electromagnetic fields and DNA damage
Research
RF Safe Research Library
Jan 1, 2009
This review discusses the comet assay and summarizes research on non-ionizing EMF exposure and DNA/chromosomal damage. It describes both positive and negative findings across studies, noting no consistent overall pattern for radiofrequency radiation (RFR). The authors nonetheless conclude that under certain exposure…