Acute exposure to 27.12 MHz radiofrequency electromagnetic field disrupts blood-brain barrier integrity via eNOS activation and occludin down-regulation.
Research
RF Safe Research Library
Jan 1, 2025
This animal study examined 27.12 MHz RF-EMF exposure for 30, 180, or 360 minutes in female Wistar rats and assessed markers related to blood-brain barrier integrity. The authors report increased eNOS expression after 30 minutes without oxidative stress or inflammation, alongside decreased occludin expression. Longer…
Effect of 900 MHz Electromagnetic Field Exposure During Different Trimesters of Pregnancy on TRPM2-Mediated Ferroptosis and Neurotoxicity in the Trigeminal Ganglion of Rats: Protective Role of Ferrostatin-1.
Research
RF Safe Research Library
Jan 1, 2025
This animal study examined prenatal 900 MHz EMF exposure during different pregnancy trimesters and measured oxidative stress, inflammatory, apoptotic, and TRPM2-related markers in rat offspring trigeminal ganglia at postnatal day 28. The abstract reports increased oxidative stress/lipid peroxidation, inflammation,…
Extremely low-frequency electromagnetic fields facilitate proliferation and functional differentiation in spinal neural stem cells.
Research
RF Safe Research Library
Jan 1, 2025
This in vitro study examined extremely low-frequency electromagnetic field (ELF-EMF) effects on spinal cord-derived neural stem cells (NSCs) from adult mice. The authors report that ELF-EMFs increase NSC proliferation and self-renewal with upregulation of Sox2. They also report activation of T-type calcium channels,…
Extremely low-frequency electromagnetic field (ELF-EMF) enhances mitochondrial energy production in NARP cybrids.
Research
RF Safe Research Library
Jan 1, 2025
This in vitro study examined ELF-EMF effects in normal cybrids and NARP cybrids carrying a pathogenic MT-ATP6 mtDNA mutation mixture. ELF-EMF did not alter mtDNA copy number or the wild-type-to-mutant mtDNA ratio, but it increased mtDNA transcription and multiple markers of mitochondrial function in NARP cybrids,…
Inflammasome Activation and IL-1β Release Triggered by Nanosecond Pulsed Electric Fields in Murine Innate Immune Cells and Skin
Research
RF Safe Research Library
Jan 15, 2024
This animal and in vitro study examined whether nanosecond pulsed electric fields (200-ns pulses) activate inflammasome signaling after electroporative damage. The authors report ASC aggregation, caspase-1 activation, and IL-1β release in murine macrophages, dendritic cells, and mouse skin in vivo. They also report…
Electromagnetic fields ameliorate hepatic lipid accumulation and oxidative stress: potential role of CaMKKβ/AMPK/SREBP-1c and Nrf2 pathways.
Research
RF Safe Research Library
Jan 1, 2023
This animal study examined EMF exposure in a mouse high-fat diet model of NAFLD. The authors report that EMF exposure reduced hepatic lipid accumulation and markers of oxidative stress, alongside changes consistent with activation of CaMKKβ/AMPK and Nrf2-related signaling. The abstract concludes EMF could be a…
Magnetofection of miR-21 promoted by electromagnetic field and iron oxide nanoparticles via the p38 MAPK pathway contributes to osteogenesis and angiogenesis for intervertebral fusion.
Research
RF Safe Research Library
Jan 1, 2023
This study describes a magnetofection system using electromagnetic field (EMF) co-stimulation with iron oxide nanoparticles (IONPs) to deliver miR-21. The authors report increased transfection efficiency and promotion of osteogenesis and angiogenesis in BMSCs and HUVECs, with downstream application to…
Structural and Functional Neural Correlates in Individuals with Excessive Smartphone Use: A Systematic Review and Meta-Analysis
Research
RF Safe Research Library
Dec 5, 2022
This systematic review and meta-analysis synthesized observational MRI studies comparing excessive smartphone users with regular users. It reports smaller brain volumes in excessive smartphone users, especially in subcortical regions, and notes that the association was more pronounced in adolescents than adults. It…
Treatment with Pulsed Extremely Low Frequency Electromagnetic Field (PELF-EMF) Exhibit Anti-Inflammatory and Neuroprotective Effect in Compression Spinal Cord Injury Model.
Research
RF Safe Research Library
Jan 1, 2022
This animal study tested a pulsed extremely low frequency electromagnetic field (PELF-EMF) SEQEX device in a mouse compression spinal cord injury model. Mice received 4 hours of daily exposure for two months starting 2 hours after injury. The study reports reduced inflammatory and astrocyte activation markers,…
Exposure to long-term evolution radiofrequency electromagnetic fields decreases neuroblastoma cell proliferation via Akt/mTOR-mediated cellular senescence.
Research
RF Safe Research Library
Jan 1, 2021
This in vitro study examined LTE RF-EMF exposure effects on SH-SY5Y neuroblastoma cells. The authors report reduced cell proliferation and a G0/G1 cell-cycle delay after 1760 MHz exposure at 4 W/kg for 4 hr/day over 4 days. They report no marked DNA damage signal (γH2AX) and no apoptosis activation, but increased…
Activation of matrix metalloproteinases and FoxO3a in HaCaT keratinocytes by radiofrequency electromagnetic field exposure.
Research
RF Safe Research Library
Jan 1, 2021
This in vitro study exposed HaCaT human keratinocytes to 1760 MHz RF-EMF (SAR 4.0 W/kg; 2 h/day for 4 days). The authors report increased intracellular ROS and increased expression/activity of several matrix metalloproteinases, along with increased phosphorylation of FoxO3a and ERK1/2. Cell growth/viability and…
Synergistic effect of electromagnetic fields and nanomagnetic particles on osteogenesis through calcium channels and p-ERK signaling.
Research
RF Safe Research Library
Jan 1, 2021
This in vitro and animal study evaluated whether extremely low frequency EMF exposure (1 mT, 45 Hz, 8 h/day) combined with Fe-based nanomagnetic particles enhances osteogenesis. The authors report no detectable cytotoxicity and increased osteogenic markers/proteins with combined NMP + EMF exposure, alongside…
Effects of electromagnetic fields treatment on rat critical-sized calvarial defects with a 3D-printed composite scaffold.
Research
RF Safe Research Library
Jan 1, 2020
This animal and in vitro study evaluated electromagnetic fields (EMF) treatment combined with a 3D-printed PLA/HA scaffold seeded with rat bone marrow mesenchymal stem cells (BMSCs) for cranial defect repair. The authors report that EMF enhanced BMSC proliferation and osteogenic differentiation and was partly…
Lipidomic alteration and stress-defense mechanism of soil nematode Caenorhabditis elegans in response to extremely low-frequency electromagnetic field exposure.
Research
RF Safe Research Library
Jan 1, 2019
This animal (nematode) study exposed Caenorhabditis elegans to 50 Hz, 3 mT extremely low-frequency EMF and assessed multi-omics responses. The authors report significant lipidomic, proteomic, and transcriptomic alterations, including increased triacylglycerols. Pathway analyses suggest disrupted lipid metabolism,…
T-Cell Differentiation to T Helper 9 Phenotype is Elevated by Extremely Low-Frequency Electromagnetic Fields Via Induction of IL-2 Signaling.
Research
RF Safe Research Library
Jan 1, 2019
This in vitro study examined 60 Hz extremely low-frequency EMF exposure (0.8 mT) on CD4 T cells under Th9-polarizing conditions. The authors report increased Th9-associated responses including IL-9 secretion, transcription factor changes (including early GATA3), and increased STAT5/STAT6 phosphorylation alongside…
Impact of Long-Term RF-EMF on Oxidative Stress and Neuroinflammation in Aging Brains of C57BL/6 Mice.
Research
RF Safe Research Library
Jan 1, 2018
This animal study exposed 14-month-old C57BL/6 mice to 1950 MHz RF-EMF (SAR 5 W/kg) for 8 months. The authors report that aging was associated with increased oxidative damage, DNA damage, apoptosis, and glial activation markers in brain tissue. Long-term RF-EMF exposure did not change these markers in aged mice and…
Wireless control of cellular function by activation of a novel protein responsive to electromagnetic fields.
Research
RF Safe Research Library
Jan 1, 2018
This study reports the identification of an electromagnetic perceptive gene (EPG) from glass catfish and its expression in mammalian cells, neuronal cultures, and rat brain. The authors report that magnetic/EMF stimulation remotely increased intracellular calcium in EPG-expressing cells and neurons. They also report…
Extremely low frequency electromagnetic fields promote mesenchymal stem cell migration by increasing intracellular Ca and activating the FAK/Rho GTPases signaling pathways in vitro.
Research
RF Safe Research Library
Jan 1, 2018
This in vitro study exposed human bone marrow-derived mesenchymal stem cells to extremely low frequency EMF (7.5–70 Hz, 1 mT) generated by Helmholtz coils. The authors report increased MSC migration across all tested frequencies. Mechanistically, the effect is described as dependent on intracellular calcium and…
Activation of TLR signalling regulates microwave radiation-mediated impairment of spermatogenesis in rat testis.
Research
RF Safe Research Library
Jan 1, 2018
This rat study examined whether microwave radiation affects Toll-like receptor (TLR) signaling in testis. The authors report increased TLR2-5 expression and elevated pro-inflammatory cytokines (IL-1β, IL-6, TNF-α) after microwave exposure. Key phosphorylated components of TLR signaling (including NF-κB p65) were also…
Enhancement of osteogenic differentiation of rat adipose tissue-derived mesenchymal stem cells by zinc sulphate under electromagnetic field via the PKA, ERK1/2 and Wnt/β-catenin signaling pathways.
Research
RF Safe Research Library
Jan 1, 2017
This in vitro study examined rat adipose-derived mesenchymal stem cells exposed to zinc sulphate in the presence of an ELF electromagnetic field (50 Hz, 20 mT). The authors report increased osteogenic differentiation markers (gene expression, ALP activity, and calcium levels) under combined ZnSO4+EMF conditions,…
Electromagnetic Fields Act Similarly in Plants as in Animals: Probable Activation of Calcium Channels via Their Voltage Sensor
Research
RF Safe Research Library
Jan 1, 2016
This narrative review proposes that low-intensity microwave/lower-frequency EMFs activate plasma membrane calcium channels in animals, increasing intracellular calcium and triggering downstream signaling including oxidative stress pathways. It further suggests that EMF actions in terrestrial multicellular plants are…
Microwave frequency electromagnetic fields (EMFs) produce widespread neuropsychiatric effects including depression
Research
RF Safe Research Library
Jan 1, 2016
This 2016 narrative review proposes that non-thermal microwave/lower-frequency EMFs act primarily through activation of voltage-gated calcium channels (VGCCs), with calcium channel blockers reported to block EMF effects. It summarizes animal, occupational, and epidemiological literature and reports that exposures…
Microwave to near-infrared conversion with a millimeter-scale wireless laser for activating molecular transducers.
Research
RF Safe Research Library
Jan 1, 2016
This conference paper reports an engineering approach to convert wireless microwave power into near-infrared light using a miniaturized, wirelessly powered laser. The device is described as millimeter-scale and capable of being powered through >1 cm of tissue. The abstract focuses on enabling in vivo activation of…
Exposure to 900 MHz electromagnetic fields activates the mkp-1/ERK pathway and causes blood-brain barrier damage and cognitive impairment in rats
Research
RF Safe Research Library
Mar 19, 2015
This animal study exposed 108 male Sprague-Dawley rats to 900 MHz EMF (1 mW/cm2) or sham for 14 or 28 days (3 h/day). The authors report that 28-day exposure was associated with impaired spatial memory, BBB permeability damage, and ultrastructural changes in hippocampus and cortex. They also report increased mkp-1…
[Modulation of Ca(2+)-Dependent Proteiolysis under the Action of Weak Low-Frequency Magnetic Fields].
Research
RF Safe Research Library
Jan 1, 2015
This animal/model-organism study examined whether weak low-frequency magnetic fields tuned to a proposed parametric resonance for calcium ions affect Ca(2+)-dependent proteolysis. It reports time-dependent modulation of Ca(2+)-dependent proteinase activity in fish and whelk and states that a minimal exposure time for…