Archive

This paper describes an in vitro gene transfection technique termed water-in-oil droplet electroporation, using an intense DC electric field to deform a droplet between electrodes. The deformation can cause an instantaneous short circuit, during which transient membrane pores are reported to form and permit exogenous…

This in vitro study examined whether electromagnetic fields (EMFs) influence chondrogenic differentiation of bovine mesenchymal stem cell pellets, with and without the inflammatory cytokine IL-1β. The authors report EMFs had limited effects on chondrogenesis in the absence of inflammation, but in the presence of…

This study develops and tests a theoretical model of how HeLa cells aggregate when exposed to a uniform AC electric field. Model-derived aggregation times and predicted string-length growth rates matched numerical simulation, and experiments observed substantial clustering in the chamber. The work is framed as…

The paper reviews and analyzes mechanisms for resonance action of extremely weak alternating magnetic fields (40 nT) combined with weak static fields (40 μT), tuned to cyclotron frequencies. It references prior reports of narrow resonant peaks in electrical conductivity of aqueous solutions and biomedical effects in…

This study tested noninvasive 13.56 MHz radiofrequency electric fields as a treatment adjunct for pancreatic ductal adenocarcinoma in cell lines and mouse xenograft models. The authors report RF exposure caused cytotoxicity in PDAC cells via autophagy (not apoptosis) and that normal pancreatic ductal epithelial cells…

This in vitro study exposed newborn rat calvarial osteoblasts to a 3.9 mT static magnetic field for varying durations (0–4 hours). The authors report increased cell proliferation and markers of osteoblast differentiation and mineralization versus control, including higher ALP activity, calcium content, and…

This in vitro FTIR study examined human haemoglobin aqueous solutions exposed to a 200 mT static magnetic field for 3 and 7 hours, with and without the disaccharides sucrose and trehalose. The authors report spectral changes (amide A decrease and reduced CH(3) stretching) in haemoglobin dissolved in bi-distilled…

This in vitro study exposed newborn rat calvarial osteoblasts to 50 Hz sinusoidal electromagnetic fields at 1.8 mT for different daily exposure times (0.5–3.0 h). The authors report inhibited osteoblast proliferation at longer exposure times, alongside increased differentiation markers (ALP activity) and…

This in vitro study examined how cortical neuron morphology and cell type relate to responses under subthreshold and suprathreshold uniform electric fields in rat motor cortex slices. The authors report that morphology correlates with subthreshold somatic polarization and that layer V pyramidal neurons are predicted…

This in vitro study reports that shortwave (MHz-range) RF exposure can induce strong cytotoxicity in EGFR-overexpressing human cancer cells when those cells have internalized cetuximab-targeted gold nanoparticles. Control nanoparticles showed minimal uptake and substantially lower cytotoxicity under RF exposure. An…

This paper describes an exposure and microscopy system designed to continuously observe living cells during exposure to modulated RF-EMF similar to mobile phone signals. Using protocols where the same cells serve as controls before and after exposure, the system was validated with endocytosis measurements. Under 900…