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Effects of 700MHz radiofrequency radiation (5G lower band) on the reproductive parameters of female Wistar rats

Research RF Safe Research Library Jan 1, 2025

This animal study examined short- and long-term 700 MHz (lower-band 5G) radiofrequency exposure in female Wistar rats, comparing control, sham, and exposed groups. It reports no DNA damage and no change in estrous cycle length, but increased ovarian oxidative stress markers in exposed animals. Long-term exposure was…

Possible effects of RF EMR on contextual fear conditioning, hippocampal perivascular space, apoptosis and adrenal gland microarchitecture in rats

Research RF Safe Research Library Jan 1, 2025

This animal study exposed male Wistar rats to 900 MHz RF-EMR from a mobile phone for 1 hour daily over four weeks and assessed behavior and tissue changes. The authors report altered contextual fear conditioning-related behavior in exposed rats. Histological assessments indicated apoptosis and enlarged perivascular…

Repeated Head Exposures to a 5G-3.5 GHz Signal Do Not Alter Behavior but Modify Intracortical Gene Expression in Adult Male Mice

Research RF Safe Research Library Jan 1, 2025

This animal study examined repeated asymmetrical head exposure to a 5G-modulated 3.5 GHz signal in adult male mice for six weeks. It reports no significant changes in locomotion, anxiety, or object-based memory performance under the tested conditions. However, it found statistically significant but limited cortical…

5G Radiofrequency Exposure Reduces PRDM16 and C/EBP � mRNA Expression, Two Key Biomarkers for Brown Adipogenesis

Research RF Safe Research Library Jan 1, 2025

This animal study exposed juvenile and young adult Wistar rats to 5G (3.5 GHz) or 2G (900 MHz) radiofrequency fields (1.5 V/m) for 1–2 weeks and measured brown adipose tissue-related gene expression by RT-qPCR. The abstract reports significant downregulation of PRDM16 and C/EBP mRNA after 5G exposure, while…

The CB1R of mPFC is involved in anxiety-like behavior induced by 0.8/2.65 GHz dual-frequency electromagnetic radiation

Research RF Safe Research Library Jan 1, 2025

This animal study reports that dual-frequency RF EMR exposure (0.8/2.65 GHz, 4 W/kg) induced anxiety-like behavior in mice. It also reports reduced CB1R and endocannabinoid levels in the mPFC and altered endocannabinoid system markers in the BLA. CB1R overexpression or knockdown in the mPFC reportedly decreased or…

Histomorphometry and Sperm Quality in Male Rats Exposed to 2.45 GHz Wi-Fi

Research RF Safe Research Library Jan 1, 2025

This animal study exposed adult male rats to 2.45 GHz Wi‑Fi from an active router for 4 or 24 hours daily over eight weeks and assessed reproductive organ histology and sperm parameters. The authors report histological changes in testes and epididymis, multifocal atypical hyperplasia in seminal vesicles, reduced…

Extremely low frequency magnetic field distracts zebrafish from a visual cognitive task

Research RF Safe Research Library Jan 1, 2025

This animal study trained adult zebrafish to perform a conditioned avoidance response to a visual cue. The visual cue was presented alone or together with an extremely low frequency sinusoidally changing magnetic field (0.3 Hz) at 0.015 mT or 0.06 mT. The abstract reports that the 0.06 mT magnetic field condition…

Sensation of electric fields in the Drosophila melanogaster larva

Research RF Safe Research Library Jan 1, 2025

This animal study reports that Drosophila melanogaster larvae can sense electric fields and exhibit robust electrotaxis toward the cathode in controlled environments. The authors identify head-tip sensory neurons required for this behavior and report calcium-imaging evidence that Gr66a-positive neurons encode field…

An experimental study on the effect of non-ionizing electromagnetic fields on honey bees.

Research RF Safe Research Library Jan 1, 2025

This experimental study investigated whether non-ionizing EMF exposure affects honey bees and honey yield. Colonies were randomized into five experimental/control groups, with experimental hives exposed to 2.4 GHz (Wi‑Fi band) and 50 Hz (high-voltage line) fields and controls placed far from EMR sources. The study…

Effects of electromagnetic field emitted by a 90 kHz WPT system on the cognitive functions and neuronal excitation of mice.

Research RF Safe Research Library Jan 1, 2025

This animal study examined whether EMF from a 90 kHz magnetic-coupling resonant wireless power transfer (WPT) system affects mouse cognition and hippocampal neuronal excitability. Mice were exposed for 2, 4, or 8 weeks and assessed with a Novel Object Recognition test and dentate gyrus patch-clamp recordings. The…

Acute exposure to 27.12 MHz radiofrequency electromagnetic field disrupts blood-brain barrier integrity via eNOS activation and occludin down-regulation.

Research RF Safe Research Library Jan 1, 2025

This animal study examined 27.12 MHz RF-EMF exposure for 30, 180, or 360 minutes in female Wistar rats and assessed markers related to blood-brain barrier integrity. The authors report increased eNOS expression after 30 minutes without oxidative stress or inflammation, alongside decreased occludin expression. Longer…

Effect of ELF-EMF on cognitive functions, analgesia, and oxidative stress in rats with PTZ-induced epilepsy.

Research RF Safe Research Library Jan 1, 2025

This animal study tested repeated 50 Hz, 5 mT ELF-EMF exposure in rats with and without PTZ-induced epilepsy. The authors report changes in learning/short-term memory in the EMF+PTZ group versus PTZ alone and increased analgesia latency in EMF-exposed groups. PTZ increased oxidative stress (TOS) in brain regions, and…

Effect of 900 MHz Electromagnetic Field Exposure During Different Trimesters of Pregnancy on TRPM2-Mediated Ferroptosis and Neurotoxicity in the Trigeminal Ganglion of Rats: Protective Role of Ferrostatin-1.

Research RF Safe Research Library Jan 1, 2025

This animal study examined prenatal 900 MHz EMF exposure during different pregnancy trimesters and measured oxidative stress, inflammatory, apoptotic, and TRPM2-related markers in rat offspring trigeminal ganglia at postnatal day 28. The abstract reports increased oxidative stress/lipid peroxidation, inflammation,…

Can Theta Burst Electromagnetic Fields Disrupt Learning in Planaria? Evidence of Impaired Fear-Conditioned Responses.

Research RF Safe Research Library Jan 1, 2025

This animal study tested whether low-intensity theta burst patterned electromagnetic fields (TBEMF; 1 μT; bursts at 100 Hz) affect fear-related learning in planaria using a T-maze with aversive light. The light-only group showed behavioral changes consistent with learning to avoid the aversive arm, while TBEMF…

Melatonin and omega-3 neuroprotection in prenatal rat spinal cord exposed to 900 MHz electromagnetic field.

Research RF Safe Research Library Jan 1, 2025

This animal study examined prenatal 900 MHz EMF exposure (2 hours/day for 21 days) in pregnant rats and assessed spinal cord outcomes in male offspring at postnatal day 35. Volume-based stereological measures (GM/WM ratios and total spinal cord volume) did not differ significantly between groups. Microscopy reported…

Possible effects of melatonin and omega-3 on the obesity-related hypothalamic nuclei of the electromagnetic field-exposed offspring rats: a stereological and immunohistochemical analysis.

Research RF Safe Research Library Jan 1, 2025

This animal study examined whether prenatal exposure to a 900 MHz electromagnetic field affects obesity-related hypothalamic nuclei in male rat offspring and whether melatonin or omega-3 modifies these effects. The EMF-exposed group showed fewer neurons in the arcuate nucleus, stronger NPY immunoreactivity, and…

Prophylactic Effects of Radiofrequency Electromagnetic Field on Pulmonary Ischemia-Reperfusion via HIF-1α/eNOS Pathway and BCL2/BAX Signaling.

Research RF Safe Research Library Jan 1, 2025

This animal study tested whether RF-EMF exposure modifies lung ischemia-reperfusion injury in male Wistar rats. IR increased histologic injury, inflammatory/apoptotic markers, oxidative stress indices, and Hif-1α/eNOS/BAX while decreasing BCL2. The IR + RF-EMF group reportedly improved across measured parameters, and…

Validation of Electromagnetic Field Sensor Performance Through Porcine Skulls: Implications for Neurostimulation and Recording Techniques.

Research RF Safe Research Library Jan 1, 2025

This animal validation study tested a Mu-metal-shielded helmet system with proprietary induction sensor stimulators for non-invasive recording and stimulation of neural electromagnetic activity through porcine skulls. The authors report that EMFs penetrated the swine skull and that sensor performance remained…

Electromagnetic field stimulation for long-term mobility assessment after sciatic nerve injury.

Research RF Safe Research Library Jan 1, 2025

This animal study evaluated electromagnetic field (EMF) stimulation as a treatment after sciatic nerve injury in male Sprague-Dawley rats. Rats received low-intensity (60–100 G; 6–10 mT) or high-intensity (140–200 G; 14–20 mT) EMF exposure for 2 hours/day over 4 weeks. The abstract reports improved motor/mobility…

Corrigendum to "Effects of radiofrequency electromagnetic field (RF-EMF) exposure on male fertility: A systematic review of experimental studies on non-human mammals and human sperm in vitro" [Environ. Int. 185 (2024) 108509]

Research RF Safe Research Library Jan 1, 2025

This 2025 corrigendum relates to a systematic review of experimental RF-EMF exposure studies on non-human mammals and human sperm in vitro. The text reports an upgrade to high certainty for the key endpoint that male RF-EMF exposure causes a significant reduction in pregnancy rate when exposed males are mated. It…

Effects of radiofrequency electromagnetic field exposure on cancer in laboratory animal studies, a systematic review

Research RF Safe Research Library Jan 1, 2025

This systematic review evaluated RF-EMF exposure and cancer outcomes in experimental animals, including 52 studies (20 chronic cancer bioassays), using narrative synthesis due to substantial heterogeneity. The review reports no or minimal evidence of RF-EMF-related cancer outcomes for many organ systems, but reports…

Inflammasome Activation and IL-1β Release Triggered by Nanosecond Pulsed Electric Fields in Murine Innate Immune Cells and Skin

Research RF Safe Research Library Jan 15, 2024

This animal and in vitro study examined whether nanosecond pulsed electric fields (200-ns pulses) activate inflammasome signaling after electroporative damage. The authors report ASC aggregation, caspase-1 activation, and IL-1β release in murine macrophages, dendritic cells, and mouse skin in vivo. They also report…

The Impact of Mobile Phone Electromagnetic Waves on the Neurons and Blood Brain Barrier Integrity in the Chick Embryo

Research RF Safe Research Library Jan 1, 2024

This animal study exposed chick embryos to electromagnetic waves from a mobile phone and compared them with unexposed controls. Electron microscopy on days 10 and 15 reported neuronal and cerebellar cellular alterations in the exposed group, including features described as apoptosis and mitochondrial swelling. The…

Role of radio-frequency electromagnetic waves in causing oxidative stress

Research RF Safe Research Library Jan 1, 2024

This animal study exposed fertilized chick eggs to a nearby 1800 MHz mobile phone that was called repeatedly (50 minutes/day) and assessed embryos at days 10 and 15. The exposed group reportedly showed mitochondrial abnormalities in liver, brain, and heart tissues on electron microscopy, along with increased HSP70 in…

Experimental Study of Animal Behavior under the Influence of the Electromagnetic Field of the 5G Mobile Communication Standard

Research RF Safe Research Library Jan 1, 2024

This pilot animal study exposed mature male Wistar rats to 4.9 GHz electromagnetic fields described as 5G-standard, for 15 days (2 hours/day) in a semi-anechoic shielded chamber. Open-field testing found no clear behavioral differences between unmodulated and modulated exposures. Statistically significant behavioral…

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