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5G Radio-Frequency-Electromagnetic-Field Effects on the Human Sleep Electroencephalogram: A Randomized Controlled Study in CACNA1C Genotyped Volunteers
This randomized, double-blind, sham-controlled study tested whether CACNA1C rs7304986 genotype modifies sleep EEG responses to 5G RF-EMF exposure. The authors report a genotype-by-exposure interaction, with 3.6 GHz exposure in T/C carriers associated with a faster NREM sleep spindle center frequency versus sham. The abstract also notes longer sleep latency in T/C compared with T/T carriers, and concludes that genetically susceptible groups may show differential physiological responses to 5G RF-EMF.
In-Situ Measurements of Radiofrequency Electromagnetic Fields Measurements Around 5G Macro Base Stations in the UK
This exposure assessment performed RF spot measurements in line-of-sight to 56 active 5G macro base stations across 30 publicly accessible UK locations. Power density was measured across 420 MHz–6 GHz under multiple scenarios (background, streaming, downlink speed test, and extrapolated SS-RSRP decoding). Reported total RF and 5G-specific levels were within 1998 ICNIRP public reference levels, with 4G downlink contributing most of the measured exposure.
Exposure to 26.5 GHz, 5G modulated and unmodulated signal, does not affect key cellular endpoints of human neuroblastoma cells
This in vitro study examined whether 26.5 GHz millimeter-wave exposure (continuous wave and 5G-modulated) affects key cellular endpoints in human neuroblastoma cells. Cells were exposed for 3 hours at SAR 1.25 W/kg using a reverberation-chamber system, with assessments including cell cycle and DNA damage. The study reports no effects from exposure alone or when combined with the oxidant menadione, while noting that additional studies across varied conditions are needed.
Histomorphometric study of thyroid tissue in juvenile rats exposed to 5G electromagnetic fields
This animal study examined thyroid histomorphometry in juvenile male Wistar rats after 2 weeks of 5G EMF exposure (3.5 GHz, 1.5 V/m). Exposed rats showed larger follicle and colloid areas and a significantly lower Thyroid Activation Index, which the authors interpret as thyroid hypoactivity. The authors suggest this may represent a potential health risk and call for further work including hormone assays and mechanistic studies.
Rapid Deployment of 5G Wireless Communication and Risk Assessment on Human Health: Quid Novi?
This review discusses the rapid deployment of 5G and the associated debate about potential human health impacts from EMF exposure, particularly at 3.5–26 GHz including millimeter waves. It emphasizes limited published studies in these exposure ranges and highlights EU-funded initiatives and research consortia aimed at closing knowledge gaps. The authors state that guidelines are generally considered adequate at present, but argue that uncertainties—especially regarding long-term exposure—support continued research and precautionary approaches.
5G-exposed human skin cells do not respond with altered gene expression and methylation profiles
This in vitro study exposed human skin cells (fibroblasts and keratinocytes) to 5G-band electromagnetic fields for 2 hours and 48 hours using a fully blinded design. Exposures were up to ten times permissible limits, with sham exposure as a negative control and UV exposure as a positive control. The study reports that observed gene expression and DNA methylation differences were minor and consistent with random variation, supporting no detectable EMF-related effect under the tested conditions.
Assessing RF EMF exposure in multiple microenvironments across ten European countries with a focus on 5G
This exposure assessment measured environmental and auto-induced RF-EMF across more than 800 microenvironments in ten European countries, with a focus on 5G-related bands. Non-user environmental exposure was reported to be below international guideline values and similar to prior European research, while induced traffic substantially increased measured exposure, especially in uplink scenarios. The study also reports systematic differences by setting (cities vs villages) and by national precautionary limit policies.
5G EMF Exposure at 3.6 GHz in Greece Using Data From Frequency-Selective Monitoring Sensors
This exposure-assessment study analyzed two years of continuous frequency-selective monitoring from 13 sensors in the five largest cities in Greece, focusing on the 3.6 GHz 5G band. It reports a gradual increase in 3.6 GHz environmental EMF levels over time and greater variability than legacy cellular bands, with 30-minute averaging reducing observed fluctuations. Despite the upward trend and variability, all measurements were reported to remain well below Greek and ICNIRP reference levels, and the authors emphasize the value of continuous monitoring as 5G deployment expands.
Deduction of Extrapolation Factors in Realistic Scenarios for In-Situ Assessment of 5G Base Stations
This conference paper examines extrapolation factors used for in-situ EMF exposure assessment of 5G base stations in realistic indoor and outdoor scenarios. Using both frequency-selective and code-selective measurement approaches under varying traffic conditions, it reports substantial variability in extrapolated exposure estimates driven largely by antenna radiation patterns. Outdoor environments showed more stable extrapolation than indoor environments, highlighting challenges for reliable exposure assessment when antenna patterns and network configurations are not well characterized.
Effects of Mobile Electromagnetic Exposure on Brain Oscillations and Cortical Excitability: Scoping Review
This scoping review evaluates evidence on mobile phone electromagnetic exposure and its effects on brain oscillations and cortical excitability in healthy individuals. Across 78 EEG studies (and 2 TMS studies), the authors report that exposure may be linked to changes in neural activity, including increased amplitudes in several EEG bands and possible changes in cortical silent period. However, substantial methodological inconsistency across studies limits firm conclusions, and the review highlights limited evidence for 5G/mmWave exposures and argues for precaution and potential guideline revision.
Assessing EMF Exposure in Greek Urban and Suburban Areas During 5G Deployment: A Focus on 5G EMF Levels and Distance Correlation
This exposure assessment reports 400 ground-level electric field measurements in Greek urban and suburban areas during 5G deployment. It finds that 4G contributes most to overall measured EMF exposure, while 5G currently contributes less. The study reports an inverse relationship between 3.5 GHz EMF levels and distance from 5G base stations, with urban areas showing higher levels than suburban areas.
Impact of expectancy on fatigue by exposure to the fifth generation of mobile communication signals
This randomized sham-controlled study in 21 healthy participants tested whether routine-level 5G RF-EMF exposure affects fatigue and EEG power, while manipulating expectancy via correct, false, or no information about exposure order. The study reports no change in EEG power with real versus sham exposure. However, self-reported fatigue varied with the conveyed information about being exposed, suggesting an expectancy/psychological priming effect on symptom reporting.
Effects of 700MHz radiofrequency radiation (5G lower band) on the reproductive parameters of female Wistar rats
This animal study examined short- and long-term 700 MHz (lower-band 5G) radiofrequency exposure in female Wistar rats, comparing control, sham, and exposed groups. It reports no DNA damage and no change in estrous cycle length, but increased ovarian oxidative stress markers in exposed animals. Long-term exposure was associated with ovarian histopathological alterations, while estradiol and progesterone stayed within normal ranges and testosterone increased slightly but significantly.
RF-EMF exposure assessment with add-on uplink exposure sensor in different microenvironments in seven European countries
This exposure assessment study introduces a cost-efficient add-on sensor attached to a smartphone to quantify auto-induced uplink RF-EMF transmission across 100–6000 MHz in multiple microenvironments. Activity-based surveys were conducted in seven European countries under non-user, maximum downlink, and maximum uplink scenarios. Reported power levels were lowest for non-user scenarios and higher during active use, with variation by country, urbanization, and setting. The authors frame the work as supporting future epidemiological research and planned validation against other tools.
The Effect of Proximity Sensor & Grip Sensor Use on Specific Absorption Rate (SAR) in Smartphones
This engineering study examined how smartphone proximity and grip sensors affect SAR during LTE and 5G NR operation in a 3D measurement environment. The abstract reports that enabling these sensors reduces SAR relative to being turned off, with reductions varying by sensor and frequency. The authors attribute the reduction to sensor-driven power management and transmission power adjustment.
Repeated Head Exposures to a 5G-3.5 GHz Signal Do Not Alter Behavior but Modify Intracortical Gene Expression in Adult Male Mice
This animal study examined repeated asymmetrical head exposure to a 5G-modulated 3.5 GHz signal in adult male mice for six weeks. It reports no significant changes in locomotion, anxiety, or object-based memory performance under the tested conditions. However, it found statistically significant but limited cortical gene expression changes (<1% of expressed genes), including enrichment for glutamatergic synapse-related genes and lateralized differences involving mitochondrial genome-encoded genes. The authors caution that potential health risks from these intracortical transcriptomic modifications should not be downplayed and note uncertainties about longer exposures and other populations.
5G Radiofrequency Exposure Reduces PRDM16 and C/EBP � mRNA Expression, Two Key Biomarkers for Brown Adipogenesis
This animal study exposed juvenile and young adult Wistar rats to 5G (3.5 GHz) or 2G (900 MHz) radiofrequency fields (1.5 V/m) for 1–2 weeks and measured brown adipose tissue-related gene expression by RT-qPCR. The abstract reports significant downregulation of PRDM16 and C/EBP mRNA after 5G exposure, while UCP1-dependent thermogenesis markers were not significantly changed at the transcriptional level. The authors interpret these findings as a potential partial disruption of brown adipocyte differentiation and raise EMF safety concerns, while calling for further confirmatory research.
Experimental Study of Animal Behavior under the Influence of the Electromagnetic Field of the 5G Mobile Communication Standard
This pilot animal study exposed mature male Wistar rats to 4.9 GHz electromagnetic fields described as 5G-standard, for 15 days (2 hours/day) in a semi-anechoic shielded chamber. Open-field testing found no clear behavioral differences between unmodulated and modulated exposures. Statistically significant behavioral changes occurred in both exposed and control animals, which the authors attribute to changes in the environmental electromagnetic background, making causal interpretation uncertain.
Effects of radiofrequency field from 5G communication on fecal microbiome and metabolome profiles in mice
This animal study exposed adult male C57BL/6 mice to a 4.9 GHz radiofrequency field for three weeks (1 hour/day) and compared them with a sham group. The abstract reports altered fecal microbiome composition with reduced diversity in the RF group, along with 258 significantly differentially abundant fecal metabolites. The authors conclude that 4.9 GHz RF exposure is associated with changes in gut microbiota and metabolic profiles and call for further EMF safety research.